摘要
通过48 h单一毒性试验和正交试验,研究了邻苯二甲酸二甲酯(DMP)、邻苯二甲酸二乙酯(DEP)、邻苯二甲酸二正丁酯(DBP)和邻苯二甲酸二异辛酯(DEHP)4种邻苯二甲酸酯类化合物暴露对斑马鱼胚胎发育的毒性效应。结果表明:这些化合物对斑马鱼胚胎发育有明显的抑制作用,可以造成胚胎发育畸形甚至死亡,具有特定最敏感的毒理学终点;4种邻苯二甲酸酯(PAEs)单独作用时毒性大小顺序依次为DBP>DEHP>DEP>DMP;在4种PAEs对斑马鱼胚胎发育的混合暴露正交试验中,DBP和DEHP存在交互作用,对其致死毒性和发育毒性影响最大;DBP虽然对斑马鱼胚胎的致死毒性和发育毒性影响不明显,但DBP可导致胚胎发育的严重畸形。
In order to assess the effects of phthalic acid esters（PAEs） on aquatic environment and specials,toxicity of Dimethyl phthalate（DMP）,Diethyl phthalate（DEP）,Dibutyl phthalate（DBP）and Di-（2-etylhexyl） phthalate（DEHP）to embryonic development of zebrafish（Danio rerio） was examined by means of 48 h exposure based on single and multicomponent orthogonal tests.Results showed that PAEs remarkably inhibited the embryonic development of zebrafish and caused embryo abnormality,even death.The most sensitive toxicological endpoints to four different congeners of PAEs had been identified.The toxicity of 4 PAEs to embryonic development of zebrafish was ranked as followed： DBPDEHPDEPDMP.In the orthogonal toxicity experiment,joint effects of DBP and DEHP were the key factors for increasing lethal ratio and developmental toxicity of PAEs.For DBP,results revealed that it had little effects on the lethal and developmental toxicity comparing with other components,but it can cause serious deformity in the combined toxicity experiment.
出处
《中山大学学报（自然科学版）》
CAS
CSCD
北大核心
2010年第5期101-106,112,共7页
Acta Scientiarum Naturalium Universitatis Sunyatseni
基金
国家自然科学基金资助项目(U063300640673062)
科技部公益研究专项资助项目(2004DIB1Z-029)
关键词
邻苯二甲酸酯
斑马鱼
胚胎毒性
联合毒性
phthalic acid esters
zabrafish
embryo toxicity test
combined toxicit
