摘要
目的:探讨邻苯二甲酸二(2-乙基己)酯(DEHP)对小鼠肺细胞DNA的损伤作用。方法:以生理盐水作为阴性对照组,用不同浓度DEHP(5、25、125、625μmol/L)对小鼠肺细胞进行体外染毒,应用单细胞凝胶电泳技术检测肺细胞DNA的断裂程度。结果:当DEHP的浓度为125和625μmol/L时,肺细胞尾部DNA百分含量、尾矩和Olive尾矩与阴性对照组相比均升高,差异具有统计学意义(P<0.05)。结论:DEHP在体外可造成小鼠肺细胞的DNA断裂损伤。
OBJECTIVE：To studyDNA damage induced by di （2-ethylhexyl） phthalate （DEHP） to mice lung cells.METHODS：The negative control group was exposed to saline. Extracted mice lung cells were exposed to different concentrations of DEHP（5,25,125,625μmol/L） and single cell gel electrophoresis was used to test the DNA breakage degree.RESULTS：Compared with the control group,tail DNA percentage,tail moment and Olive tail moment of 125 μmol/L and 625μmol/L DEHP-treated groups were increased significantly （P〈0.05）.CONCLUSION： DEHP could induce DNA breakage of mice lung cells in vitro.
出处
《癌变．畸变．突变》
CAS
CSCD
2014年第5期386-388,共3页
Carcinogenesis,Teratogenesis & Mutagenesis
