摘要
为了获得何首乌悬浮细胞系、研究何首乌细胞的液体培养和二苯乙烯苷的代谢路径,首先要获得何首乌愈伤组织.文中以何首乌根和茎为外植体,通过正交试验筛选根和茎诱导愈伤组织的适宜培养基,结果表明,在26℃和暗培养条件下,MS培养基中添加1mg/L2,4-二氯苯氧乙酸和0.4mg/L萘乙酸最适宜诱导出愈伤组织,诱导率可以达到96.7%.通过HPLC测定了根和茎愈伤组织的二苯乙烯苷含量,分别为1.5mg/g和0.4mg/g.
In order to obtain the suspension cell line of Polygonum multiflorum Thunb.and investigate the suspension culture and stilbene glycoside metabolic pathways,callus must be obtained.In this paper,the root and the stem of Polygonum multiflorum Thunb.were used as explants to acquire the most suitable callus induction medium via orthogonal tests.It is found that the root and the stem are most appropriately induced on the MS medium containing 1mg/L 2,4-dichlorophenoxyacetic acid and 0.4mg/L naphthaleneacetic acid at 26℃ in dark,with the induction rate being up to 96.7%.Moreover,HPLC results indicate that the contents of stilbene glycoside in root and stem callus are respectively 1.5 and 0.4mg/g.
出处
《华南理工大学学报（自然科学版）》
EI
CAS
CSCD
北大核心
2010年第9期147-150,共4页
Journal of South China University of Technology(Natural Science Edition)
基金
广东省自然科学基金资助项目(06019716)
关键词
何首乌
愈伤组织
高效液相色谱
二苯乙烯苷
Polygonum multiflorum Thunb.
callus
high-pressure liquid chromatography
stilbene glycoside
