摘要
通过组织切片和活细胞涂片对斗嫁Cellana grata(Gould)性腺和生殖细胞进行观察,研究雌性性腺特征及不同发育阶段雌性生殖细胞的形态特点与分布状况。观察与分析表明:(1)斗嫁雌性滤泡由卵原细胞及不同发育时期的初级卵母细胞填充,成熟的初级卵母细胞位居滤泡腔中央区域;(2)性细胞发育不同步,属分批产卵类型;(3)卵子发生中,细胞、核及核仁大小变化的总体趋势是随着发育进程而逐渐增大,常见1-2个核仁;(4)卵质的酸碱特性变化与卵黄的出现相关;(5)成熟卵母细胞大小约为(120-140)μm×150μm,外被约5-12μm厚的胶质膜;(6)胶质膜的均一性、厚度、酸碱性随卵发育而变化,胶质膜与卵膜之间出现间隙可以作为卵开始成熟的标志,而胶质膜由嗜酸性转化为嗜碱性可以作为卵成熟即将排放的标志。
The gonad and gametocyte of Cellana grata （Gould） were observed under microscope by puncture and historical section to study the characteristic of female gonad and the morphology and distribution of oogametes at different developing stages. The results showed that： （1） the female folliculus of C. grata were composed of oogoniums and oocytes at different developing stages, and matured oogametes were located around the central region of follicle; （2） The development of gametocytes was asynchronous and spawning came up on successive type. （3） The gametocytes and its nuclei and nucleolus trended to enlarge during the oogenesis with the maturity of the gonad, and 1—2 nucleolus appeared. （4） Characteristic of acido-and baso-philic of oocytoplasma changed with coming up of oocytoyolk. （5） Matured egg were in range of about （120—140） μm × 150 μm in size, which were rounded by an jelly coat with thickness 5—12 μm. （6） The uniformity and acidity of jelly coat were changeable according to the developing stages of gametocyte. It was the signal of oogoniums becoming mature that gaps appeared between jelly coat and the oocyte membrane; and it was another signal of oogoniums being matured and went to spawn that acidophilic of the oogoniums transformed to basophilic.
出处
《水生生物学报》
CAS
CSCD
北大核心
2010年第5期913-921,共9页
Acta Hydrobiologica Sinica
基金
国家科技支撑计划项目2007BAD29B03
珠海万山区海洋与渔业局项目(B04034)资助
关键词
斗嫁
卵子发生
雌性性腺
胶质膜
Cellana grata （Gould）
Oogenesis
Female gonad
Jelly coat
