摘要
目的:研究大蒜新素对巨细胞病毒(CMV)感染鼠的免疫抑制性细胞因子TGF-β1和IL-10的影响,探讨大蒜新素的抗CMV机制。方法:建立非免疫抑制性鼠巨细胞病毒(MCMV)全身播散性感染小鼠模型;双抗体夹心ELISA法检测感染后1,3,7,14,28,45,60,75,90,120d正常对照组、病毒感染组和药物治疗组小鼠脾细胞培养上清中TGF-β1和IL-10水平的时序性变化。结果:MCMV感染后,TGF-β1和IL-10分泌均显著升高,并呈现出急慢性期两次高峰的特点。大蒜新素治疗对MCMV感染鼠各时间点TGF-β1水平均有下调作用,且对其急性期分泌高峰的抑制更明显。大蒜新素治疗对IL-10的急性期分泌高峰影响不明显,却显著下调慢性期IL-10的分泌高峰。结论:大蒜新素能显著抑制MCMV感染后TGF-β1和IL-10分泌,进而增强机体细胞免疫功能而有利于机体清除MCMV病毒,这可能是大蒜新素抗CMV的另一作用机制。
Objective：To study the influence of allitridin on the level of immunosuppressive cytokines TGF-β1 and IL-10 in mouse cytomegalovirus（MCMV）infected mice,and to find the mechanisms of allitridin against CMV.Methods：A disseminating infected murine model was established without using immunosuppressant.TGF-β1 and IL-10 level in spleen cell culture supernatants were measured in normal control group,MCMV infected group and allitridin treated group by doubleantibody sandwich ELISA at 1,3,7,14,28,45,60,75,90,and 120 day（s）post infection.Results：The levels of TGF-β1and IL-10 elevated remarkably after MCMV infection,and both showed the feature of two crests in the time-concentration curve.Allitridin could reduce the increasing secretion of TGF-β1in all time points,especially on the first crest.And allitridin had no evident effects on the first crest of IL-10 level,but it could suppress IL-10 secretion significantly on the second crest.Conclusion：Allitridin could inhibit the secretion of TGF-β1 and IL-10 in MC-MV infected mice,which could facilitate the immunity of the host and was helpful for the clearance of MCMV.This might be another protective mechanism of allitridin against CMV.
出处
《武汉大学学报（医学版）》
CAS
北大核心
2010年第5期580-583,共4页
Medical Journal of Wuhan University
基金
国家自然科学基金资助项目(编号:30572345)
