摘要
[目的]研究猪核转录因子C/EBPβ,扩增C/EBPβ3′非翻译区(3′UTR)部分序列。[方法]以报道的猪C/EBPβ基因片段(DQ450678.1)为模板设计引物,应用3′末端快速扩增技术(3′RACE技术)进行扩增。[结果]成功克隆猪核转录因子C/EBPβ的3′UTR,并获得GenBank登录号:FJ869121。[结论]与GenBank中报道的其他哺乳动物相应序列进行比较分析,猪核转录因子C/EBPβ的3′UTR序列与人C/EBPβ基因核苷酸序列BLAST比对,同源性为93%,为克隆其基因组全长及分析其功能奠定了基础。
[Objective] To clone the 3＇terminal regions of Nuclear Transcription FactorC/EBPβ in pig using rapid amplification of cDNA ends （RACE） technique. [Method] According to the sequence of the techniques of DQ450678.1 in NCBI,3＇non-translated region（3＇UTR） was cloned using RACE. [Result] The 3＇UTR ofC/EBPβ was amplified successfully with RACE and the accession number in GenBank was given as FJ869121. [Conclusion] There was 93% nucleotide acid identity between the sequences ofC/EBPβ in swine and human in NCBI nucleotide acid database. This study laid a foundation for obtaining the full sequence and analyzing the function and structure of genome ofC/EBPβ in Sus scrofa.
出处
《安徽农业科学》
CAS
北大核心
2010年第23期12469-12470,12475,共3页
Journal of Anhui Agricultural Sciences
基金
辽宁省科技厅项目(2009408-6)
辽宁省教育厅重点实验室项目(2009S067)
