摘要
目的观察不同基质对阿萨希毛孢子菌生物膜形成能力的影响。方法在聚芳脂、聚苯乙烯、聚氯乙烯上构建阿萨希毛孢子菌生物膜,在生物膜形成过程中采用XTT法对其活性进行定量分析,倒置显微镜和扫描电镜下观察不同基质上阿萨希毛孢子菌生物膜形态特征。结果 3种基质上均能形成阿萨希毛孢子菌生物膜,且形成广泛的的生物膜。比较成熟期不同基质上形成生物膜的活性有差别(F=14.743,P<0.01),活性由高到低为聚芳脂=聚氯乙烯>聚苯乙烯。倒置显微镜和扫描电镜下观察发现聚芳脂、聚氯乙烯形成的生物膜可见孢子、菌丝、假菌丝结构,聚苯乙烯上形成以孢子为主要结构的微生物群落。结论阿萨希毛孢子菌可在聚芳脂、聚苯乙烯、聚氯乙烯上形成生物膜,但形成生物膜的能力不同。聚芳脂、聚氯乙烯比聚苯乙烯更易于真菌的黏附;且以菌丝、假菌丝为主要结构的微生物群落活力比单纯孢子的活力强。
Objective To study biofilm formation of T.asahii（T.asahii BF）on different matrix in vitro.Methods The biofilm of T.asahii was formed on PAT,polystyrene and PVC.The activity of biofolm was measured and quantitatively analyzed by XTT.Morphological characteristics of T.asahii was observed under inverted microscope and scanning electron microscope.Results Biofilm was formed extensively on PAT,polystyrene and PVC.The vitality of biofilm formed on different materials differed from strong to weak as followed：PAT=PVCpolystyrene（F=14.743,P0.01）.Yeast cells,pseudohyphas and hyphae were found on PAT and PVC,but only yeast cells were observed on polystyrene.Conclusions T.asahii could form biofilms on different support materials,but differes in ability.It＇s more easier to adhere on PAT and PVC than on polystyrene,and the vitality of pseudohyphas and hyphae is stronger than that of yeast cells.
出处
《中国真菌学杂志》
2010年第4期201-205,共5页
Chinese Journal of Mycology
基金
国家自然科学基金(30700038)
