摘要
目的:研究人晶状体上皮细胞(HLECs)雌激素受体(ER)表达的情况及中药植物雌激素异补骨脂素(ISR)对ER表达的影响,为中药植物雌激素防治老年性白内障提供实验依据。方法:本研究将ISR与HLEC共同孵育,以雌二醇(E2)作为阳性对照,再用H2O2对HLECs造成氧化损伤后,采用流式细胞术检测不同浓度的ISR对ER蛋白表达的影响。结果:正常HLECs存在ERα、β的表达;H2O2组HLECsERα、β表达明显下降,与空白组比较差异显著(P<0.01);E2和ISR高、中、低浓度组HLECs的ERα、β表达明显升高,与H2O2组比较差异显著(P<0.01);且随ISR浓度的增高ERα、β表达逐渐升高,呈明显的浓度依赖关系。结论:E2、ISR能上调经H2O2处理的HLECs的ERα、ERβ表达,并呈明显的浓度依赖关系,其抗氧化损伤作用,可能是通过上调ERα、ERβ表达实现的。
AIM： To investigate the effect of isopsoralen（ ISR） on the expression of estrogen receptor （ ER） in human lens epithelial cells （ HLECs） . METHODS： HLECs were cultured and sub-cultured in vitro. The cultured HLECs pretreated with E2 or ISR were exposed to H2O2 at the concentration of 300 μmol/L. The expression of ERα and ERβ in HLECs was analyzed by flow cytometry. RESULTS： The expression of ERα and ERβ in H2O2 group was obviously decreased as compared to control group （ P 0. 01） . The expression of ERα and ERβ in the cells treated with E2 and with ISR at the concentration of 10-5 mol/L,10-6 mol/L or 10-7 mol/L plus H2O2 was obviously increased as compared to the cells treated with H2O2 only （ P 0. 01） . A concentration-dependent effect of ISR was observed. CONCLUSION： H2O2 decreases the expression of ERα and ERβ in HLECs. E2 and ISR increase the expression of ERα and ERβ in HLECs treated with H2O2 in a concentration-dependent manner,which may account for their antioxidative effect.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2010年第9期1844-1848,共5页
Chinese Journal of Pathophysiology
基金
福建省中医药重点资助项目(NoW2260602)
