摘要
以千层金茎段为外植体进行离体培养,通过试验筛选出各阶段适宜的培养基,建立了千层金组培快繁体系。结果表明:在诱导不定芽的培养基中,MS+6-BA1mg/L的芽萌发率最高,芽体健壮;丛生芽继代增殖培养基为改良MS+6-BA1mg/L+NAA0.1mg/L+KT0.1mg/L;生根培养基为1/2MS+1号生根剂0.5mg/L+IBA0.1mg/L,生根率达100%,且根系发达;栽培基质以椰糠:腐殖土=2:1较理想,移栽成活率为89.3%。
The stem segments ofMelaleuca bracteata was used as explants, the tissue culture and rapid propagation technology system was established. The results showed that the best initiated medium was MS＋6-BA 1 mg/L, and the best proliferation medium was MS＋6-BA 1 mg/L＋NAA 0.1 mg/L＋KT 0.1 mg/L. The optimum shoots rooting medium was 1/2MS＋IBA 0.1 mg/L, with the rooting of 100% and developed roots. The survival rate of tissue culture plantlets was over 89.3% after the plantlets acclimation transplanting experiment.
出处
《中国农学通报》
CSCD
北大核心
2010年第18期247-250,共4页
Chinese Agricultural Science Bulletin
基金
福建省公益类科研院所专项"亚热带几种特色经济作物种质资源收集与保存"(2010R1101012-3)
福建省农科院青年人才创新基金"两个适合南方栽培的新型彩叶树种引进及生产技术研究"(QC2006-yys08)
关键词
千层金
组培
快繁
Melaleuca bracteata
tissue culture
rapid propagation
