摘要
以甘农1号杂花苜蓿下胚轴为材料诱导愈伤组织,将愈伤组织转移到添加不同浓度PEG6000的UM+0.1mg/LNAA+0.5mg/LKT培养基上,发生体胚,研究PEG6000对体胚发生的影响,并观察了体胚的细胞学结构。结果表明,适量的PEG6000对体胚的发生有促进作用,其中添加6%PEG6000体胚的发生率最高;石蜡切片观察得出体胚的发育经过球形胚、心形胚、鱼雷胚及子叶胚4个阶段形成一个完整的体胚。
The callus induced from the hypocotyl inMedicago varia cv.‘Gannong No.1’, was cultured in the UM medium supplied with the PEG6000 of various concentrations, NAA （0.1 mg/L） and KT （0.5 mg/L）, to induce the somatic embryos. Then the study on effect of PEG6000 on the somatic embryogenesis and observation of embryo cytological structure were carried out in this paper. The results show that, the regeneration medium with proper concentration of PEG6000 are advantageous to somatic embryogenesis, the highest frequency of somatic embryogenesis appeared in regeneration medium with 6%PEG6000; via observed the paraffin sections, A whole somatic embryo will go through four developing phases, heart-shape embryo, torpedo embryo and cotyledon-shape embryo.
出处
《中国农学通报》
CSCD
北大核心
2010年第18期63-66,共4页
Chinese Agricultural Science Bulletin
基金
国家863计划重点项目"我国地方畜禽品种优良特性的分子遗传评估及综合利用技术"(2008AA101001)
青岛农业大学校人才基金"青岛牧草生态适应性的研究"(630526)
山东省优秀中青年科学家科研奖励基金"小反刍兽疫植物疫苗初步研究"(BS2009SW010)
山东省农业良种工程牧草项目"优质高产牧草和饲料作物新品种选育"(2008LZ019-01
2008LZ019-04)
关键词
杂花苜蓿
聚乙二醇
体胚
细胞学观察
Variegated Alfalfa
PEG
somatic embryo
cytological observation
