摘要
以海带（Ｌａｍｉｎａｒｉａｊａｐｏｎｉｃａ）雌配子体克隆ＬＨ１０品系为材料，用４种植物生长激素：三十烷醇（ＴＡ）、２，４－二氯苯氧乙酸（２，４－Ｄ）、吲哚乙酸（ＩＡＡ）和腐植酸钠加以处理，对其生长发育进行了研究。结果表明，它们的最佳生长浓度分别为：ＴＡ，０．０１ｍｇ／Ｌ；２，４－Ｄ０．５ｍｇ／Ｌ；ＩＡＡ，０．５ｍｇ／Ｌ；腐植酸钠，０．５ｍｇ／Ｌ；最佳发育浓度分别为：ＴＡ，０．０１ｍｇ／Ｌ；２，４－Ｄ，１ｍｇ／Ｌ；ＩＡＡ，０．１ｍｇＬ－１；腐植酸钠，０．０５ｍｇ／Ｌ。
Intact phycobilisomes were obtained from wild type Gracilaria lemaneiformis from Qingdao Zhanshan Bay (wt), yellow mutant (ye100), 4 green mutants (gr180, gr184, gr186 and gr188), wild type G. lemaneiformis from Venezuela (lv), from South Africa (sa) and G. asiatica (ga) by sucrose gradient centrifugation method. The phycobilisomes of wt, lv, sa and ye100 were in 1.0￣1.5 mol/L sucrose layer; gr188 was in 0.75￣1.0 mol/L sucrose layer; and the other three green mutants were in 0.75 mol/L sucrose layer. For G. asiatica, there were phycobilisomes of different molecular weights, corresponding to positions of 0.75 mol/L and 1.0 mol/L, respectively. The absorption spectra of the phycobilisomes were also measured in the present paper. The absorption peaks of wt were at 499 nm, 543 nm, 568 nm and 614 nm, along with a shoulder of 650 nm. Compared with wt, the absorption spectra of ye100, lv, sa and ga were basically the same, but they were slightly different in PEB/PUB ratio and PC, APC relative content. On the other hand, the phycobilisome spectral character of green mutants (gr180, gr184, gr186 and gr188) and ga was quite different from that of wt, showing a decrease in PE and an increase in PC and APC contents, and change in phycobilisome structure. The difference of phycobilisomes among wild type and mutants of G. Lemaneiformis were more significant than that among materials from different habitats and different species of the genus.
基金
国家自然科学基金
国家高技术发展计划项目
关键词
海带
雌配子体
克隆
植物生长激素
生长发育
Gracilaria lemaneiformis
Gracilaria asiatica
pigment mutants
phycobilisome
absorption spectra
