摘要
目的探讨肝移植受者白介素(interleukin,IL)-10的表达与急性排斥反应之间的关系。方法分别用酶联免疫吸附试验(enzyme-linked immune absorbent assay,ELISA)和免疫组织化学方法检测59例肝脏移植受者外周血及肝穿刺组织中IL-10的表达情况。以有否发生急性排斥反应将受者分为急性排斥组和非排斥组;并将非排斥组按肝功能情况分为肝功能正常组与肝功能异常组,应用受试者工作特征(receiver operating characteristic,ROC)曲线分析外周血IL-10诊断急性排斥反应的诊断临界点、敏感度和特异度。结果急性排斥组外周血及肝组织中IL-10表达均显著低于非排斥组(均为P<0.05)。非排斥组中肝功能正常组与异常组之间外周血及肝组织中的IL-10表达比较差异无统计学意义(P>0.05)。IL-10诊断急性排斥反应的临界值为9.45pg/ml,敏感度为0.591,特异度为0.676。结论 IL-10的低表达提示肝脏移植受者发生急性排斥反应,该指标可作为诊断急性排斥反应的辅助指标。
Objective To explore the relationship of expression of interleukin（IL）-10 and acute rejection in patients with liver transplantation.Methods The IL-10 expression in blood samples and liver biopsies from fifty-nine patients were assayed by the enzyme-linked immune absorbent assay（ELISA）and immunohistochemistry methods.The recipients were divided into acute rejection group and non-rejection group according to whether acute rejection occurred.And non-rejection group was divided into normal group and abnormal group according to the liver function.The results were analyzed by receiver operating characteristic（ROC）curve.Results The expressions of IL-10 in serum and liver biopsies in the acute rejection group were significantly lower than that in non-rejection group（all in P〈0.05）.In the group without rejection,there was no statistical difference between the normal liver function group and abnormal liver function group.The IL-10 level at 9.45 pg/ml was considered as cut-off point to diagnose liver acute rejection with a sensitivity of 0.591 and a specificity of 0.676.Conclusion Patients with low expression of the IL-10 are prone to suffer liver acute rejection and IL-10 level can be regarded as an auxiliary diagnosis index for acute rejection.
出处
《器官移植》
CAS
2010年第5期269-271,275,共4页
Organ Transplantation
基金
青岛市科技局课题(06-2-2-6-nsh-1)
