摘要
目的探讨血管紧张素Ⅰ转化酶（ＡＣＥ）基因插入／缺失多态性与老年人２型糖尿病（ＤＭ２）及糖尿病肾病合并肾功能不全（ＲＩ）的相关情况，同时观察血清ＡＣＥ水平与ＡＣＥ基因多态性及疾病的关系。方法对病程差异无显著性的７３例ＤＭ２患者、６８例ＲＩ患者及７２例健康对照者用ＰＣＲ方法进行了ＡＣＥ基因内含子１６插入／缺失多态性的检测，用紫外分光光度法测定了血清ＡＣＥ水平。结果ＲＩ组Ｄ等位基因频率为０．５３，ＤＤ基因型频率０．３２，与对照组（分别为０．３８、０．１５）比较差异有显著性（均为Ｐ＜０．０５），ＤＤ基因型与ＩＩ基因型比较对ＲＩ的比数比为３．２９（Ｐ＜０．０１）；ＲＩ组血清ＡＣＥ水平显著高于对照组（Ｐ＜０．００１）。结论ＡＣＥ基因缺失多态性可能是老年人群ＲＩ的危险因素之一。
Objective To investigate whether the insertion/deletion polymorphism of angiotensin Ⅰ converting enzyme (ACE) gene is associated with diabetic nephropathy and complicated renal insufficiency (RI) in the aged,with type 2 diabetes mellitus without nephropathy (DM2), and to ascertain the effects of serum ACE levels and ACE gene polymorphism on the development of the diabetic nephropathy. Methods ACE genotypes were determined in 73 cases with DM 2, 68 with RI and 72 controls by polymerase chain reaction (PCR). ACE levels were measured with ultraviolet photometry. Results The increased frequencies of D allele (0 53) and DD genotype (0 32) were observed in 68 subjects with RI as compared with 72 control subjects (0 38 and 0 15 respectively, P <0 05). The odds ratio associated with DD/II genotype was 3 29 ( P <0 01) for RI. Patients with RI had higher serum ACE levels compared with controls ( P <0 001). Conclusions Deletion polymorphism of the ACE gene might be an important risk factor for progression of renal insufficiency in old age patients with diabetic nephropathy.
出处
《中华老年医学杂志》
CAS
CSCD
北大核心
1999年第2期80-83,共4页
Chinese Journal of Geriatrics
关键词
ACE
基因
糖尿病肾病
糖尿病
病理
Angiotensin converting enzyme Gene Diabetic nephropathy Diabetes mellitus, non insulin dependent
