摘要
利用梯度稀释和对峙试验的方法,从向日葵根际土壤筛选到18株对向日葵菌核病病原菌—核盘菌(Sclerotiniasclerotiorum)有拮抗效果的细菌,其中1株具有较强的拮抗效果,命名为XRK4。经过形态观察及16S rDNA序列分析,将XRK4鉴定为地衣芽孢杆菌(Bacillus licheniformis)。研究表明XRK4具有β-1,3-1,4-葡聚糖酶活性。设计引物以XRK4的基因组DNA为模板扩增得到长度为732 bp的β-1,3-1,4-葡聚糖酶的完整基因,其开放阅读框架编码243个氨基酸。XRK4可能因产生葡聚糖酶,降解病原菌细胞壁而具有拮抗活性。
Eighteen antagonistic bacteria were screened from sunflower rhizosphere,which is resistant to Sclerotinia sclerotiorum.One of the isolated strains named as XRK4 was identified as Bacillus licheniformis based on the morphologic characteristics and phylogenetic analysis of 16S rDNA.The results showed that XRK4 has β-1,3-1,4-glucanase activity.The open reading frame of β-1,3-1,4-glucanase gene is 732 bp encoding 243 amino acids.XRK4 might have antagonistic activity based on its β-1,3-1,4-glucanase gene.
出处
《生物技术通报》
CAS
CSCD
北大核心
2010年第9期138-142,153,共6页
Biotechnology Bulletin
基金
山东省优秀中青年科研奖励基金(2006BS06012)
关键词
向日葵根际
拮抗细菌
核盘菌
Β-1
3-1
4-葡聚糖酶
Sunflower rhizosphere Antagonistic bacteria Sclerotinia sclerotiorum β-1
3-1
4-glucanase
