摘要
本研究评价了从向日葵列当体内分离筛选得到的内生黏质沙雷氏菌（Serratia marcescens）LIEH 92对向日葵菌核病进行盆栽与大田防效评价,并测定了该菌株的生理特性和其在根际土与向日葵体内的定殖情况。结果表明,LIEH 92发酵液对向日葵菌核病的室内盆栽防效达73.35%,对大田根腐型菌核病和盘腐型菌核病防效分别达53.48%和38.64%。菌株LIEH 92可产生几丁质酶,并能在根际土和向日葵根、茎、叶内定殖与传导,定殖菌量达102~106 cfu/g。其在向日葵植株的根内定殖数量最大,茎中次之,叶中最少。在灭菌土中LIEH 92在根际土和向日葵根部的定殖菌量小于在自然土中其在相应部位的定殖菌量,而灭菌土中LIEH 92在向日葵茎部和叶部的定殖菌量则大于自然土中相应的定殖菌量。LIEH 92处理可提高向日葵植株PAL、POD和PPO等防御酶活性,从而诱导向日葵对菌核病的抗性。LIEH 92对向日葵菌核病具有生防潜力。
Serratia marcescens LIEH 92 was obtained from Orobanche cumana. The control efficacy of LIEH 92 against sunflower sclerotinia rot were evaluated in pot experiments and field plot experiments. The physiological characteristics of LIEH 92 and its colonization in rhizosphere soil and sunflower plants were determined. The re- sults showed that the control efficacy of fermented liquid from LIEH 92 against sunflower sclerotinia rot was 73. 35% in pot experiments, 53.48% for sunflower sclerotinia root rot and 38.64% for sunflower sclerotinia head rot in field plot experiments. The chitinase-producing by LIEH 92 could colonize and transmit in rhizosphere soil and roots, stems, leaves of sunflowers. The quantity of LIEH 92 reached 102 - 106 cfu/g, and the most was observed in roots, followed by stems and leaves. The colonizing bacterial quantity of strain LIEH 92 from rhizosphere soil and roots in sterile soil was lower than that in natural soil, while the colonizing bacterial quantity from stems and leaves in sterile soil was higher than that in natural soil. Strain LIEH 92 increased the activities of defensive en- zymes PAL, POD and PPO in sunflowers, and the resistance of the plants to Sclerotinia sclerotiorum was induced. LIEH 92 has great potential for bio-controlling sunflower sclerotinia rot.
出处
《植物保护》
CAS
CSCD
北大核心
2016年第1期73-80,共8页
Plant Protection
基金
内蒙古自治区高等学校科学技术研究一般项目(NJZY12277)
河套学院引进人才科研启动项目(HYRC2014001)
河套学院科学技术研究自然科学重点项目(HTXYZZ13001)
关键词
向日葵菌核病
黏质沙雷氏菌
防病效果
定殖动态
防御酶活性
Sclerotinia sclerotiorum of sunflower
Serratia marcescens
control efficacy
colonization dy-namics
defense enzyme activity
