摘要
目的：观察大鼠肠缺血再灌注后肾脏内源性碱性成纤维细胞生长因子（ｂＦＧＦ）和转化生长因子β（ＴＧＦβ）基因与蛋白表达水平的变化，探讨ｂＦＧＦ和ＴＧＦβ促进损伤修复的分子调控机制。方法：采用大鼠肠系膜上动脉夹闭模型，将动物随机分为假手术组、缺血４５分钟组、再灌注６小时组及再灌注２４小时组。ｂＦＧＦ和ＴＧＦβ基因表达用原位杂交方法检测，蛋白表达用免疫组织化学过氧化物酶标记的链酶卵白素（ＳＰ）染色方法测定。结果：肠缺血再灌注损伤后肾内源性ｂＦＧＦ和ＴＧＦβ基因和蛋白的表达水平均明显增高，于再灌注６小时达峰值，２４小时有所下降。结论：肠缺血再灌注损伤诱导了内源性ｂＦＧＦ和ＴＧＦβ的表达。
Objective:To investigate renal endogenous expression of basic fibroblast growth factor (bFGF) and transforming growth factor β (TGFβ) after intestinal ischemiareperfusion injury and its protective effect on renal injury.Methods:48 male Wistar rats were subjected to superior mesenteric artery occlusion followed by reperfusion.Animals were randomly divided into shamoperated,ischemia 45 minutes,6 and 24 hours postreperfusion groups.bFGF and TGFβ mRNA expression were examined by in situ hybridization and their protein expression by immunohistochemistry respectively.Results:bFGF and TGFβ expression were upregulated after ischemiareperfusion injury,peaking at 6 hours and decreasing at 24 hours after reperfusion.Conclusions:The results indicate that acute renal injury can induce endogenous bFGF and TGFβ expression,which may be a compensatory protective response to kidney repair.
出处
《中国危重病急救医学》
CSCD
1999年第4期203-205,共3页
Chinese Critical Care Medicine
基金
国家杰出青年科学基金
关键词
肠缺血
再灌注损伤
bFGF
TGFΒ
基因表达
修复
ischemiareperfusion injury
intestinal\ \ basic fibroblast growth factor
transforming growth factor β\ \ gene expression\ \ repair
