摘要
目的:探讨2型糖尿病(T2DM)患者基质金属蛋白酶-9基因-1562C/T(MMP-9-1562C/T)多态性与糖尿病肾病(DN)及DN不同病期的关系。方法:将150例T2DM患者按DN诊断标准分为DN组与非糖尿病肾病(NDN)组,DN组分为微量白蛋白尿期、临床白蛋白尿期和肾功能不全期。另择52例健康人作为正常对照(NC)组。应用限制性片段长度多态性(RFLP)分析各组基因型。结果:DN组的糖化血红蛋白(HbA1c)、收缩压(SBP)、肌酐(Cr)、尿素氮(BUN)、尿微量白蛋白排泄率(UAER)值高于NDN、NC组(P<0.01)。DN组与NDN组、NC组之间基因型分布差别均有统计学意义(P<0.01)。T2DM中CC、CT、TT各基因型DN发生率依次递减,其中TT基因型组DN发生率低于CC基因型组(P<0.01)。携T等位基因者发生DN的风险是携C等位基因者的0.47倍(P<0.01,95%CI0.29～0.75)。MMP-9-1562C基因型、HbA1c、TG、TC、Cr、BUN、SBP是DN发生的危险因素;DN组内微量白蛋白尿期、临床白蛋白尿期和肾功能不全期TT基因型与T等位基因频率随肾功能减退而降低(P<0.05或P<0.01)。结论:MMP-9-1562C/T基因多态性与DN发生有关,T等位基因是DN患者的保护基因。
Objective： To explore the relationship between matrix metalloproteinase-9 （MMP-9） gene -1562C/T and diabetic nephropathy （DN） with different stage. Methods： One hundred and fifty patients with type 2 diabetes （T2DM） were divided into DN group and diabetes without DN （NDN） group. Patients in DN group included microamount albuminuria, clinical alubuminuria and renal inadequacy stages. Fifty-two healthy people were selected as normal control （NC） group. The genotypes were assessed by restriction fragment length polymorphism （RFLP）. Results： The values of glycosylated hemoglobin（HbA1c）, systolic pressure （SBP）, creatinine（Cr）, blood urea nitrogen （BUN）, urine albumin excretion rate（UAER） were significantly higher in DN group than those of NDN or NC group（P 0.01）.There was significant difference in frequencies of allele or genotype of polymorphism C/T between DN group and NDN or NC group （P 0.01）. DN incidence rates were decreased gradually by turns in T2DM patients containing genotypes of CC, CT or TT. The incidence of DN was lower in TT genotype groups than that of CC genotype groups （P 0.01）. The T allele carriers had 0.47 times risk for increased incidence of DN compared with that of C allele carriers （P 0.01,95%CI 0.29～0.75）. The MMP-9 -1562C, HbA1c, TG, TC, Cr, BUN and SBP were the independent risk factors of DN. The frequencies of TT genotype and T allele decreased with renal hypofunction in microamount albuminuria, clinical albuminuria and renal inadequacy stage of DN （P 0.05 or P 0.01）. Conclusion： The polymorphism of MMP-9-1562C/T is associated with the development of DN. T allele is the protective gene of DN.
出处
《天津医药》
CAS
北大核心
2010年第8期644-647,737,共5页
Tianjin Medical Journal
基金
安徽省卫生厅基金资助项目(项目编号:2002A040)
