摘要
目的建立一种特异、敏感、简便的疟疾聚合酶链反应(PCR)快速诊断方法。方法针对疟原虫SSU rRNA基因序列设计3条分别具有属、种特异性的引物,通过多重PCR反应扩增间日疟原虫和恶性疟原虫不同大小的DNA片段。结果 19份恶性疟原虫血样均扩增出长度为400 bp的特定基因片段,16份间日疟原虫扩增出长度为300 bp的特定基因片段,20份健康人群对照血样经PCR扩增均为阴性。结论该多重PCR检测系统具有高效、敏感、特异、稳定、简便等特点,适宜于大批量样品同时检测,对疟疾的快速诊断、鉴别混合感染有较高的应用价值。
Objective To establish a specific,sensitive and simple method for malaria diagnosis and species differentiation.Methods A common upper primer and two species-specific lower primers were designed according to the small subunit ribosomal DNA（SSU rDNA）fragments sequences of two Plasmodium species.The multiplex PCR system was established and applied to detect P.vavix and P.falciparum in the stock blood samlpes of clinically confirmed patients.Results DNA fragments of 300 and 400 base pairs were successfully amplified by the multiplex PCR from the genomic DNA of 35 samples.The assay gave negative result for 20 blood specimens of healthy persons.Conclusion The multiplex PCR system is able to test and differentiation malaria infection.
出处
《中国预防医学杂志》
CAS
2010年第8期822-825,共4页
Chinese Preventive Medicine
关键词
疟疾
SSU
RRNA基因
多重聚合酶链反应
混合感染
Malaria
SSU rRNA gene
Multiplex polymerase chain reaction
Species differentiate diagnosis
