摘要
目的将全自动显微图像扫描采集系统应用于血吸虫病病原学诊断,用以替代传统的人工镜检,提高粪片血吸虫镜检效率。方法采用单盲法全自动显微图像扫描采集系统和5名专业人员传统镜检读片,各检读20张Kato-Katz片样片,并计数读得的阳性粪片;统计每个镜检员检读每张粪片所需的时间。比较2种方法的读片速度、敏感度、特异度、准确度。结果图像系统诊断的整体准确度为99.00%,高于传统镜检准确度的91.00%(P<0.05);敏感度和特异度分别为98.57%和100%,高于传统镜检法的90.00%和93.33%(P均<0.05)。结论全自动显微图像扫描采集系统功能强大,扫描速度快,操作简便快捷,在血吸虫读片方面优于传统镜检读片,适于在基层推广使用。
Objective To apply the automatically microscopic scanning system in the etiological diagnosis of schistosomiasis,as an alternative to the traditional manual microscopy detection,and improve the detection rates and efficiency of examinations of fecal slides.Methods A single-blind method was used throughout the process of both the automatic microscopic scanning system and traditional manual microscopy reading.Each of five professionals read 20 Kato-Katz manure piece samples.The detection results and time for each sample were recorded.The two methods were compared by the film reading speed,sensitivity,specificity,and accuracy.Results The overall accuracy of the diagnosis of schistosomiasis of the automatically microscopic scanning image system was 99.00%,higher than that of the traditional manual microscopy detection（91.00%）（P 0.05）;the sensitivity and specificity of the image system were 98.57% and 100%,respectively,and also higher than those of the traditional manual microscopy method（90.00% and 93.33%）（all P 0.05）.Conclusions The automatically microscopic scanning system is powerful,the scanning speed is fast,and the operation is easy.Its speed,accuracy,sensitivity and specificity are superior to those of the conventional manual microscopy;therefore,it is suitable for the etiological diagnosis of schistosomiasis at the grassroots＇ level.
出处
《中国血吸虫病防治杂志》
CAS
CSCD
2012年第6期659-662,共4页
Chinese Journal of Schistosomiasis Control
基金
江苏省卫生厅科研项目(X201101)
关键词
血吸虫病
显微图像
病原学诊断
Schistosomiasis
Microscopic image
Etiological diagnosis
