摘要
目的:研究PI3K抑制剂LY294002对SGC7901胃癌细胞增殖凋亡及相关调控因子的影响。方法:MTT法测定细胞增殖,流式细胞术AnnexinV-FITC/PI染色法检测细胞凋亡率,RT-PCR检测NAG-1、Skp2mRNA的表达情况。结果:LY294002可抑制SGC7901的生长,呈时间-剂量依赖性,细胞表现出形态学改变;经10、30、50μmo.lL-1的LY294002作用12h的凋亡率依次为(12.7±0.42)%、(36.5±0.45)%、(64.1±0.79)%,明显高于空白对照组的(2.3±0.36)%(均P<0.01);LY294002可上调NAG-1mRNA表达,下调Skp2mRNA表达。结论:LY294002可改变胃癌细胞株SGC7901Skp2和NAG-1基因的表达,抑制细胞增殖,诱导细胞凋亡,其机制可能与其调控NAG-1、Skp2mRNA表达水平有关。
Objective：To explore the influence of PI3K inhibition by LY294002 on the proliferation and correlated regulatory factors of gastric carcinoma cell SGC7901.Methods：The effect of LY294002 on the proliferation of gastric cancer cell were evaluated by MTT assays.Flow cytometry analysis was used to determine the apoptosis rate.RT-PCR was applied to measure the expression of NAG-1（Non-steroidal anti-inflammatory drug activated gene-1）and Skp2（S-phase kinase associated protein2）mRNA.Results：Administered with LY294002,the proliferation of gastric cancer cell decreased in dose-and time-dependent manners.The cell apoptosis rates after the treatment with 10,30,50 μmol·L^-1 LY294002 for 12 h were（12.7±0.42）%,（36.5±0.45）%,（64.1±0.79）% respectively,which were higher than those in control group（2.3±0.36）%（P〈0.01）.The expression of Skp2 mRNA was down-regulated while that of NAG-1 mRNA was up-regulated.Conclusion：LY294002 could inhibit proliferation and induce apoptosis of SGC7901 cells,possibly through the regulation of Skp2 and NAG-1 expressions.
出处
《现代医学》
2010年第3期230-234,共5页
Modern Medical Journal
