摘要
目的应用飞行质谱分析技术对小鼠肠道菌群蛋白组分进行分析,探讨肠道菌群定性、定量和蛋白组学分析方法。方法实验分为抗生素干预组与正常对照组。抗生素干预组小鼠用400 mg/ml头孢曲松钠灌服,每次0.2 ml,每日2次,间隔6 h,连续8 d。收集2组小鼠盲肠内容物菌群细胞,采用飞行质谱分析技术进行肠道菌群蛋白组分分析,采用传统培养法对2组优势菌群进行定量分析。结果抗生素干预组的肠道菌群菌细胞的有效蛋白峰出峰的数量与正常对照组比对差异有统计学意义,2组小鼠的肠道菌群蛋白成分有明显的不同。抗生素干预组的肠道优势菌群数量显著低于正常对照组。结论应用飞行质谱分析技术可以快速明显区分抗生素干预小鼠与正常小鼠的肠道菌群,对肠道菌群研究和临床合理应用抗生素具有指导意义。
Objective To explore the qualitative and quatitative methods of intestinal flora and proteomic analysis in mice by flight Mass Spectrometry.Method Mice were divided into two groups,the antibiotic intervention group and the normal group.The mice in Antibiotic intervention group were fed with 400 mg/ml ceftriaxone sodium,0.2 ml each time,2 times per day at 6 h interval for 8 days.Bacterial cells from cecal contents of the two groups were collected for analysis of protein components of intestinal flora by flight mass spectrometry.Quantitative analysis of dominant bacteria was done by traditional culture.Result Compared with the normal group,the number of effective protein peak of intestinal flora bacteria cell in Antibiotic intervention group was significantly different.The number of Intestinal dominant bacteria of antibiotic intervention group was significantly lower than that of control group.Conclusion Application of flight mass spectrometry technology can quickly differentiate the intestinal flora between antibiotics intervention group and the normal group,having deeply significance on the intestinal flora research and clinical rational use of antibiotics.
出处
《中国微生态学杂志》
CAS
CSCD
2010年第8期673-675,共3页
Chinese Journal of Microecology
基金
国家自然基金(30670045)
国家"973"科学计划项目(2007CB513006)
国家"863"科学计划项目(2007A10Z356)
关键词
飞行质谱分析
肠道菌群
蛋白组分分析
Flight mass spectrometry
Intestinal flora
Analysis protein components
