摘要
利用发光菌毒性测试技术,研究了5种重金属化合物HgCl2、Pb(NO3)2、Cd(NO3)2、K2Cr2O7、NaAsO2对3种常见发光菌种——青海弧菌Q67、明亮发光杆菌502、费氏弧菌的毒性效应差异.同时,对发光抑制率和金属化合物浓度进行线性回归分析后计算了EC50值(半数效应浓度值),并对比了不同菌种对各金属化合物的敏感度差异及特点.结果表明,5种重金属对青海弧菌Q67的毒性大小顺序为HgCl2>Cd(NO3)2>NaAsO2>Pb(NO3)2>K2 Cr2 O7;对明亮发光杆菌502的毒性大小顺序为:HgCl2>NaAsO2>Pb(NO3)2>Cd(NO3)2>K2 Cr2 O7;对费氏弧菌的毒性大小顺序为:HgCl2>Pb(NO3)2>Cd(NO3)2>NaAsO2>K2Cr2O7.研究结果对利用发光菌毒性测试技术测定不同废水污染时菌种的选择具有指导作用,在未知污染物的情况下应选用几种菌种同时检测,这样可以保证检测结果的可靠性.
The inhibiting toxicity of five heavy metal compounds-HgCl2,Pb（NO3） 2,Cd（NO3） 2,K2 Cr2 O7 and NaAsO2-on the luminescence of three kinds of luminescent bacteria-Vibrio qinghaiensis sp.-Q67,Photobacterium phosphoreum and Vibrio fischeri-was investigated by use of the Microtox test.The EC50 s（Median Effective Concentrations） of the five metals were measured based on linear regression analysis.The results showed that the toxic effects of the heavy metal compounds on the three kinds of luminescent bacteria were different.The toxicity order among the five heavy metal compounds to Vibrio qinghaiensis sp.-Q67 was HgCl2 Cd（NO3） 2 NaAsO2 Pb（NO3） 2 K2 Cr2 O7.To Photobacterium phosphoreum the toxicity order was HgCl2 NaAsO2 Pb（NO3） 2 Cd（NO3） 2 K2 Cr2 O7,and to Vibrio fischeri the order was HgCl2 Pb（NO3） 2 Cd（NO3） 2 NaAsO2 K2 Cr2 O7.The research results are an important guide to determine the risk of different wastewaters.The choice of different luminescent bacteria depends on the kind of wastewater tested by the Microtox test.If unknown pollutants are present in the wastewater,several kinds of luminescent bacteria should be chosen to ensure the reliability of results by Microtox test.
出处
《环境科学学报》
CAS
CSCD
北大核心
2010年第9期1787-1792,共6页
Acta Scientiae Circumstantiae
关键词
发光菌
重金属化合物
敏感度
废水
luminescent bacteria
heavy metal compounds
sensitivity differences
wastewater
