摘要
目的探讨手霉素对白血病干细胞(LSC)的细胞毒作用。方法采用MTT法检测手霉素对LSC的细胞增殖抑制作用,采用Annex-inⅤ-FITC法检测LSC细胞凋亡情况并采用流式细胞术分析细胞周期。结果随着手霉素浓度的增高,LSC细胞增生抑制率明显增高,细胞凋亡率也逐渐增高,且阻滞于G2/M期的细胞比率明显增加。结论手霉素通过阻滞LSC于G2/M期,促进凋亡而抑制其增长,而对正常造血干细胞(HSC)无细胞毒作用。
Objective To probe into the cytotoxic effect of manumycin on leukemic stem cell （LSC）.Methods MTT method was used to detect the inhibitory effect of manumycin on LSC proliferation.Annexin Ⅴ-FITC method was adopted to determine LSC apoptosis to analyze cell cycle by flow cytometry.Results With the increase of manumycin concentrations,the inhibitory rate of LSC proliferation had obvious increase,the apoptosis rate also gradually increased,and the cell rate arrested in G2/M phase was significantly enhanced.Conclusions Manumycin could promote apoptosis and inhibit LSC prolieration by arresting LSC in G2/M phase,but has no cytotoxic effect on normal hemopoietic stem cell （HSC）.
出处
《中国老年学杂志》
CAS
CSCD
北大核心
2010年第16期2257-2258,共2页
Chinese Journal of Gerontology
基金
吉林省科技发展计划项目(20090454)
关键词
法尼基转移酶抑制剂
手霉素
白血病干细胞
细胞增生
细胞凋亡
Farnesyltransferase inhibitors
Manumycin
Leukemic stem cell （LSC）
Cellular proliferation
Apoptosis
