摘要
目的 制备经聚乙二醇修饰的壳聚糖纳米粒（PEG/CS NP）,并负载表柔比星（EPI）,研究载表柔比星的壳聚糖纳米粒（PEG/CS-EPI NP）体外释药性能.方法 应用阴离子凝聚法制备PEG/CS-EPI NP,透射电镜观察纳米粒的形态特征,激光粒度分析仪测定粒径大小,紫外分光光度法测定纳米粒的载EPI量,动态透析法考察载EPI纳米粒的体外释放特性.结果 当壳聚糖与三聚磷酸钠质量比为6∶1,壳聚糖与EPI质量比为8∶1时,制备的PEG/CS-EPI NP呈圆形或椭圆形,分散性良好,平均粒径（322.1±14.4）nm,载EPI量为（13.9±1.1）%,包封率（74.2±1.8）%,72 h累积释药率达（82.0±2.1）%.结论 采用阴离子凝聚法制备的PEG/CS-EPI NP形状规则、粒度分布均匀,具有较高包封率和较好缓释性能.
Objective To prepare epirubicin-loaded chitosan nanoparticles （PEG/CS-EPI NP）modified by polyethylene glycol and study the drug release behavior in vitro. Methods PEG/CS-EPI NP were prepared by ionic gelation technique. The particle size and morphology were determined by laser scattering and transmission electron microscopy respectively. The drug loading and releasing behavior of PEG/CS-EPI NP in vitro were examined by ultraviolet spectrophotometry and dynamic dialysis. Results NPs were round or ovoid spheres,evenly distributing,with a mean diameter of （322.1 ± 14.4） nm. The loading capacity and encapsulation efficiency were estimated to be （ 13.9 ± 1.1 ）% and （74.2 ± 1.8）%,respectively. The 72 h cumulative release was （82.0±2.1）% of the loaded dose. Conclusion Spherical and well distributed PEG/CS NP with high encapsulation efficiency and good performance of sustained drug release can be prepared by ionic gelation technique.
出处
《中华生物医学工程杂志》
CAS
2010年第2期108-111,共4页
Chinese Journal of Biomedical Engineering
基金
基金项目：广东省医学科研基金（A2007680）
关键词
表柔比星
壳聚糖
纳米粒
聚乙二醇
缓释
Epirubicin
Chitosan
Nanoparticles
Polyethylene glycol
Sustained release
