摘要
目的观察并比较小鼠树突状细胞（ＤＣ）和巨噬细胞对外源性抗原的内吞路径的差异。方法制备小鼠骨髓ＤＣ和腹腔巨噬细胞，然后让ＤＣ和巨噬细胞与辣根过氧化物酶（ＨＲＰ）５ｎｍ胶体金共孵育１０分钟后再培养０～１２０分钟，进行酸性磷酸酶细胞化学反应和组织相容性抗原（ＭＨＣ）Ⅱ免疫胶体金标记，最后在电镜下观察５ｎｍ胶体金的细胞内运行路径。结果ＤＣ内吞ＨＲＰ５ｎｍ胶体金１０分钟后再培养３０分钟，大部分５ｎｍ胶体金进入ＭＨＣⅡ隔室（ＭＩＣ）中，只有极少数酸性磷酸酶阳性的溶酶体内含少量５ｎｍ胶体金。而与ＤＣ显著不同的是巨噬细胞摄入的大部分５ｎｍ胶体金进入了溶酶体，还有部分５ｎｍ胶体金进入ＭＩＣ中。内吞后再培养６０分钟，ＤＣ内仍有许多５ｎｍ胶体金，而巨噬细胞内已很少见到５ｎｍ胶体金。结论同一种外源性抗原被ＤＣ和巨噬细胞内吞后，在这两类细胞中的运行路径明显不同。内吞后３０分钟，巨噬细胞摄入的大部分抗原进入溶酶体，而ＤＣ则将大部分抗原送入ＭＩＣ，这可能与ＤＣ独特的抗原提呈功能有关。另外，巨噬细胞对抗原的清除能力比ＤＣ强得多。
Objective To compare the endocytic routes of exogenous antigen in murine dendritic cells (DC) and macrophages.Methods Murine bone marrow derived DC and peritoneal macrophages were pulsed with HRP 5nm colloidal gold for 10 minutes and chased for 0～120 minutes in culture medium. Intracellular distribution of 5 nm colloidal gold was explored by means of cellular enzymatic chemistry of acidic phosphatase and MHC Ⅱ cellular immunochemistry under electron microscope. Results After 10 minutes pulse with HRP 5 nm colloidal gold and then 30 minutes of chase, most of HRP 5 nm colloidal gold internalized by DC entered into MHC Ⅱ positive MIICs, but a little entered into acidic phosphatase positive lysosomes. In contrast to DC, most of lysosomes of macrophages were accessed by HRP 5 nm colloidal gold, and a small portion of HRP 5 nm colloidal gold entered into MIICs. After 60 minutes of chase, 5nm colloidal gold could hardly be seen within macrophages, whereas most of 5 nm colloidal gold retained in DC. Conclusion The endocytic route of exogenous antigen in DC apparently differs from that in macrophages. Most of antigens taken by macrophages enter into lysosomes within 30 minutes. In the case of DC, most of internalized antigens enter into MIICs, which may be related to their unique antigen presenting function . In addition, macrophages seem to have more powerful capacity of scavenging exogenous antigen than DC.
出处
《中华医学杂志》
CAS
CSCD
北大核心
1999年第3期166-169,共4页
National Medical Journal of China
基金
国家自然科学基金
