摘要
采用福林酚法及三氯化铝法分析红松子种皮提取物中多酚及黄酮类成分组成;通过提取物清除自由基能力、抑制脂蛋白过氧化能力及金属离子鳌合能力的测定来评价其抗氧化活性。结果显示,红松子种皮提取物富含多酚及黄酮类成分,质量分数分别为262及174mg/g。红松子种皮提取物对卵黄脂蛋白的过氧化过程具有显著的抑制作用,当添加提取物质量浓度0.5~1.5g/L时脂蛋白过氧化抑制率由49.27%升至95.44%,极大程度地抑制了脂蛋白的过氧化;提取物具有较好清除自由基能力,在0.16~1.6g/L范围内对2,2′-连胺-3-乙基苯并噻唑啉-6-磺酸二铵盐(ABTS)自由基的清除率由17.53%升至97.83%,红松子种皮提取物同时具有较强的金属离子鳌合能力,10g/L样品质量浓度对应的Fe2+鳌合率为57.74%。
Study on the bioactive component and antioxidant activities of extract from Pinus koraiensis Sieb.et Zucc.(PKS) seed shell, was carried out. Folin-Ciocalteau and AlCl3 methods were adopted for the analysis of polyphenol and flavonoid extracts. Radical scavenging assay, lipoprotein oxidation inhibition assay and metal ion chelation power test were adopted to detect the antioxidant activity of extract. Result showed that the contents of polyphenol and flavonoid in the extract were 262 and 174mg/g respectively. The extract has highly effective inhibition for lipoprotein oxidation, at 0.5 to 1.5g/L, the inhibitory rate was increased from 49.27% to 95.44%; the extract also has strong radical scavenging effect, the inhibitory rate for 2,2′-azido-bis(3-ethylbenzthiazoline-6-sulfonic acid)(ABTS) radical was increased from 17.53% to 97.83% at 0.16 to 1.6g/L respectively; the extract has effect on Fe2+ chelation and the chelation rate was 57.74% at 10g/L.
出处
《林产化学与工业》
EI
CAS
CSCD
北大核心
2010年第4期99-102,共4页
Chemistry and Industry of Forest Products
关键词
红松子种皮
活性成分
脂蛋白氧化
Fe2+鳌合
Pinus koraiensis Sieb.et Zucc.seed shell
active component
lipoprotein oxidation
Fe2+ chelation