摘要
盐生杜氏藻(Dunaliella salina)是已知最耐盐的真核光合生物,硝酸盐作为盐生杜氏藻氮源的主要来源,影响盐生杜氏藻的生长代谢,研究盐生杜氏藻对硝酸盐的转运吸收状况,可为生长于高盐、低盐环境中作物吸收氮的研究提供理论基础.以水杨酸法测定硝酸盐浓度,设计相关实验,结果发现盐生杜氏藻在低浓度的硝酸盐(0.2mmol/L)溶液中对硝酸盐有吸收,吸收效率随硝酸盐浓度的增大而提高;当盐浓度为2mol/L时其硝酸盐吸收效率最高,说明一定的盐浓度能诱导盐生杜氏藻对硝酸盐的吸收;经(NH_4)^+预处理过的盐生杜氏藻,在相同硝酸盐浓度下培养时其硝酸盐吸收效率比未经(NH_4)^+预处理的低.实验结果提示盐生杜氏藻存在高亲和性的硝酸盐转运蛋白.
Dunaliella salina,a unicellular green alga,known as one of the most salt-tolerant photosynthetic eukaryotic organisms,is capable to be adapted to an extremely wide range of salinities,from 0.1 to 5.5 mol/L NaCl.Nitrate is the main N source for D.salina,and influences its growth metabolism.This study can be useful for future studying nitrate metabolic pathway and regulation,and also provide theory basis for nitrate uptake by crops growing in the environment with high or low level of salinity.Salicylic acid colorimetry was used to measure nitrate concentrations in D.salina after its culture in the nitrate solutions.The results showed that at low concentration（0.2 mmol/L）of nitrate,D.salina could absorb nitrate,and the rate of nitrate uptake increased along with the increase of the nitrate concentration.D.salina achieved the highest rate of nitrate uptake when cultured in the solution with nitrate of 2 mol/L,which demonstrated that appropriate concentration of salt could induce D.salina to enhance its nitrate uptake.The rate of nitrate uptake of D.salina pretreated with （NH_4）~＋ was lower than that of control.The results suggested the existence of the high affinity nitrate transporter protein in D.salina.Fig 6,Ref 17
出处
《应用与环境生物学报》
CAS
CSCD
北大核心
2010年第4期489-492,共4页
Chinese Journal of Applied and Environmental Biology
基金
国家自然科学基金项目(Nos.30871321
30771312
30971817)
国家重点基础研究发展计划("973"计划
No.2009CB125910)资助~~
