摘要
将从深海硅藻中克隆的与氮具有高度亲和性的硝酸盐转运蛋白基因NAT3通过农杆菌介导的遗传转化方法,转入水稻品种中花11,在含30 mg/L Basta的选择培养基上筛选,获得456棵抗性植株,对NAT3目标基因检测,获得313棵阳性植株,初步证明目标基因已整合到中花11基因组中。
NAT3 gene coding protein with high nitrate combining ability cloned from Cylindrotheca fusiformis was transmitted into rice ＇zhonghua 11＇ via Agrobacterium-mediated transformation.456 regenerated rice plants with resistance were obtained by screening on the culture medium with 30 mg/L basta.313 positive transgenetic plants were gained by PCR amplification analysis targeting at NAT3 gene.It was confirmed that NAT3 gene had been integrated into the genome of ＇zhonghua 11＇.
出处
《湖北农业科学》
北大核心
2011年第24期5247-5249,共3页
Hubei Agricultural Sciences
基金
湖北省科技厅国际合作项目(2010BFA019)
