摘要
目的进一步分析正常组织来源的细胞系ＣＶ１和人大肠癌细胞系ＣＣＬ２２９的Ｇｒｐ９４的表达及其与细胞周期的关系。方法对细胞中ＤＮＡ和内质网分子伴侣Ｇｒｐ９４进行同时染色，进行双参数流式细胞术分析。结果两种细胞的Ｇｒｐ９４的表达情况相似，在细胞周期各时相中内质网分子伴侣Ｇｒｐ９４均有表达，Ｇ０＋Ｇ１期表达量较少，Ｓ期的表达量无明显增加，Ｇ２＋Ｍ期的表达量最高。结论Ｇｒｐ９４在细胞周期各时相的表达呈渐增的趋势，但在Ｓ期增加不明显，表明Ｇｒｐ９４的表达与细胞周期各时相的变化存在着密切的关系。
Objective
To analyse the relationship of the expression of molecular chaperone Grp94 ER with cell cycle.
Methods The expression of Grp94 related to the different stages was reported with Dualparameter
flow cytometry methods. Results The expression of Grp94 was found during all stages of cell
cycle.It was expressed at the lowest in G0-G1stage,a slightly higher level in S stage and the
highest in G2-Mstage. Conclusion The expression of Grp94 is related to the stage of cell cycle.
出处
《中华物理医学与康复杂志》
CAS
CSCD
1999年第2期109-110,共2页
Chinese Journal of Physical Medicine and Rehabilitation
基金
国家自然科学基金
