摘要
目的:建立高效液相-荧光检测器(HPLC-FLD)法测定乌灵胶囊(乌灵菌)中14种氨基酸含量的方法。方法:采用邻苯二甲醛和β-巯基乙醇进行柱前衍生,磷酸氢二钠缓冲液(0.1mol/L,pH=6.86)-甲醇为流动相,流速:1.0mL/min,柱温:30℃,经梯度洗脱后荧光检测器(激发波长340nm,发射波长450nm)测定氨基酸的含量。结果:14种氨基酸衍生物在40min内均能得到良好的分离和测定,各氨基酸的浓度在5～200nmol/mL范围内,线性相关系数大于0.9990。结论:该方法简单、准确,重现性好,可用于乌灵胶囊中氨基酸的含量测定。
AIM： To establish an analytical method for detecting 14 kinds of amino acid in Wuling Capsule（xylaria） by HPLC-FLD. METHODS： After being derivated in precolumn with o-phthalaldehyde and β-mercaptoethanol,the amino acids were eluted by gradient elution and were detected with a fluorescence detector （Ex = 340 nm,Em = 450 nm）. The mobile phase consisted of 0. 1 mol/L NaH2PO4 （ adjusted to pH 6. 8 by phosphoric acid） and methanol with a flow rate of 1. 0 mL/min,column temperature was set at 30 ℃. RESULTS： The deriva-tives of 14 amino acids with o-phthalaldehyde could be separated with a stable base line within 40 min. The linear range of the detection was 5-200 nmol/mL and correlation coefficients were over 0. 999 0. CONCLUSION： The method is simple,accurate and well reproduced for the determination of amino acids in Wuling Capsule.
出处
《中成药》
CAS
CSCD
北大核心
2010年第8期1358-1361,共4页
Chinese Traditional Patent Medicine
