摘要
为提高堆肥中难溶性磷的转化及堆料的降解效率,选取课题组前期筛选、驯化的4株耐高温解无机磷菌(X1,X2,X3和X4)和2株纤维素降解菌(X5和X6),采用均匀设计对不同配比的菌株进行液体发酵培养,通过对发酵培养后解磷能力的指标分析,优选复合菌配比.经均匀设计软件及DPS数据处理系统优化的结果表明,最佳的菌剂配比:φ(X1)=13.66%,φ(X2)=11.36%,φ(X3)=11.41%,φ(X4)=13.61%,φ(X5)=10.62%,φ(X6)=39.34%.回归后的配方所得解磷总量实测平均值(230.12mg/L)与理论值(228.75mg/L)基本一致.将优化的组合菌剂接种到纤维素液体发酵培养基中,培养168h后CMCase酶活性高达39.76U.
Four strains of thermo-stable inorganic phosphorus-solubilizing microorganisms （ X1,X2,X3,X4 ） and two strains of cellulose-degrading microorganisms （ X5 ,X6 ）,screened and domesticated in previous experiments,were selected and mixed at different ratios according to uniform experimental design methods,and then cultured in fermentation broth to enhance the conversion efficiency of insoluble phosphorus and degrading rate of composting materials during the compost process. The ratio of each microorganism in the complex bacteria was optimized by analysis of the indexes of solubilizing insoluble phosphorus capabilities using uniform Design Software and Data Processing System （DPS）. The results indicated that the optimum ratio of the complex bacteria was： φ（X1） = 13. 66% ,φ（ X2 ） = 11. 36% ,φ（ X3 ） = 11. 41% ,φ（ X4 ） = 13. 61% ,φ（ X5 ） = 10. 62% ,and φ（ X6 ） = 39. 34% . The phosphorus-solubilizing capability of the optimized complex bacteria was investigated,and the measured total mean value of solubilized-phosphorus was 230. 12 mg /L,which was in accordance with the theoretical value of 228. 75 mg /L by regression. The complex bacteria combination was also inoculated into cellulose fermentation broth,and the CMCase activity attained as high as 39. 76 U after 168 hours’culture.
出处
《环境科学研究》
EI
CAS
CSCD
北大核心
2010年第8期1039-1043,共5页
Research of Environmental Sciences
基金
国家自然科学基金项目(50878201)
黑龙江省自然科学基金项目(C200917)
国家水体污染控制与治理科技重大专项(2008ZX07209-007-004)
农业科技成果转化资金项目(2008GB24420470)
关键词
复合菌剂
均匀设计
解磷能力
compound bacterium
uniform design
solubilized-phosphorus capability
