摘要
Objective:To prepare folate liposome complexes of recombinant plasmid pEGFP/SDF-1/KDEL wich contained intrakine SDF-1/KDEL gene and to observe its targeting for breast cancer cells.Methods:Reverse phase evaporation method was employed for preparing folate-liposome complexeses of pEGFP/SDF-1/KDEL,so that preparation condition was optimized;and fluorometric method was taken for testing encapsulation efficiency.Particle diameter of liposome was tested by transmission electron microscopy.Complexeses encapsulated with 0.05-0.25 mg/ml calcein were got and incubated with breast cancer cell line MDA-MB-231 cells for 2 to 12 h,then dissolved them with dimethyl sulfoxide,then detected absorbance with microplate reader.Results:The optimized encapsulation condition are as follows:molecular ratio of lecithin and cholesterol was 3:1,rotary speed was 150 r/min under temperature of 43℃,and encapsulation efficiency reached 81%in this experiment.Average liposome particle diameter was 210 nm;8 h after incubation, the intake of MDA-MB-231 cells to 0.25 mg/ml compounds achieved saturation. Conclusion:The pEGFP/SDF-1/KDEL folic acid liposome complexes prepared has a high encapsulating rate,liposome particle diameters are homogeneous,which is available for targeting for breast cancer cells in vitro.
Objective： To prepare folate liposome complexes of recombinant plasmid pEGFP/SDF-1/KDEL wich contained intrakine SDF-1/KDEL gene and to observe its targeting for breast cancer cells. Methods： Reverse phase evaporation method was employed for preparing folate-liposome complexeses of pEGFP/SDF-1/KDEL, so that preparation condition was optimized; and fluorometric method was taken for testing encapsulation efficiency.Particle diameter of liposome was tested by transmission electron microscopy. Complexeses encapsulated with 0.05--0.25 mg/ml calcein were got and incubated with breast cancer cell line MDA-MB-231 cells for 2 to 12 h, then dissolved them with dimethyl sulfoxide, then detected absorbance with microplate reader. Results： The optimized encapsulation condition are as follows： molecular ratio of lecithin and cholesterol was 3：1, rotary speed was 150 r/rain under temperature of 43~C, and encapsulation efficiency reached 81% in this experiment. Average liposome particle diameter was 210 nm; 8 h after incubation, the intake of MDA-MB-231 cells to 0.25 mg/ml compounds achieved saturation. Conclusion： The pEGFP/SDF-1/KDEL folio acid liposome complexes prepared has a high encapsulating rate, liposome particle diameters are homogeneous, which is available for targeting for breast cancer cells in vitro.
基金
National Natural Science Foundation of China
grant number:30572209
