摘要
目的:明确健脾化湿方对AD小鼠AQP3表达的影响。方法:将30只小鼠分为空白对照组、空白模型组、中药模型组,每组10只。特应性皮炎模型建立后,空白模型组给予生理盐水灌胃,中药模型组给予健脾湿化方灌胃,剂量为10 mL/kg,每日2次,2周后应用荧光定量PCR法、Western blotting法检测健脾化湿方对AD小鼠皮肤组织AQP3 mRNA及蛋白的影响。结果:Western blotting检测结果显示:空白对照组、空白模型组和中药模型组AQP3/β-actin值分别为0.13±0.04,0.87±0.22和0.35±0.099。荧光定量PCR检测显示:空白对照组、空白模型组和中药模型组AQP3/β-actin值分别为0.374±0.075,1.285±0.135和0.720±0.077。结论:健脾化湿方可以改善皮肤组织中AQP3蛋白及AQP3 mRNA的表达,可能与促进皮肤的修复有关。
Objective： To determine the effects of traditional Chinese medicine of Jianpihuashi on the expression of AQP3. Methods： Thirty mice were divided into blank group, model control group and Jianpihuashi group. Atopic dermatitis models were constructed first, and then, the mice in the model control group were gavaged with saline and those in the Jianpihuashi group were gavaged with Jianpihuashi, 10 mL/kg, twice a day. The level of AQP3 was detected by RT-PCR and Western blotting. Results： The level of AQP3/β-actin in the blank group, model control group and Jianpihuashi group was 0.13±0.04, 0.87±0.22 and 0.35±0.099. RT -PCR showed the level of AQP3/β-actin in the blank group, model control group and Jianpihuashi group was 0.374±0.075,1.285±0.135 and 0.720±0.077. Conclusion： The Jianpihuashi can improve the expression of AQP3, which may relate to the repairmen of the lesion.
出处
《中国麻风皮肤病杂志》
2015年第1期18-20,共3页
China Journal of Leprosy and Skin Diseases
基金
济南市科技发展计划(编号:201221043)
