摘要
A simple and rapid capillary electrophoresis(CE) method is firstly established for the separation and determination of rutin and quercetin in Gansu particle.After a series of experiments,10 mmol·L-1 borate buffer containing 5% acetonitrile at pH 9.4 is selected as the optimum,the separation voltage and temperature are 20 kV and 25℃ respectively.In the tested concentration range,good linear relationships between the peak area and the concentration of the analytes are revealed(correlation coefficients:Rutin 0.9996 and Quercetin 0.9995).Finally,the method is successfully applied to the analysis of the two compounds in Gansu Particle sample.
A simple and rapid capillary electrophoresis（CE） method is firstly established for the separation and determination of rutin and quercetin in Gansu particle.After a series of experiments,10 mmol·L-1 borate buffer containing 5% acetonitrile at pH 9.4 is selected as the optimum,the separation voltage and temperature are 20 kV and 25℃ respectively.In the tested concentration range,good linear relationships between the peak area and the concentration of the analytes are revealed（correlation coefficients：Rutin 0.9996 and Quercetin 0.9995）.Finally,the method is successfully applied to the analysis of the two compounds in Gansu Particle sample.
出处
《化学研究与应用》
CAS
CSCD
北大核心
2010年第8期1046-1049,共4页
Chemical Research and Application
基金
西华大学重点科研基金项目(Z0913305)
关键词
槲皮素
芦丁
毛细管电泳
Quercetin
Rutin
Capillary Electrophoresis
