摘要
目的观察中药溃结饮在灌胃与灌肠2种给药途径下,对血清γ干扰素(IFN-γ)、白细胞介素-4(IL-4)和IFN-γ/IL-4比值的干预作用。方法将大鼠随机分为正常组、模型组、奥沙拉嗪钠对照组、溃结饮灌胃组和溃结饮灌肠组,共5个组。除正常组仅用普通饲料饲养外,其余各组均用三硝基苯磺酸(TNBS)/乙醇法造模,并分别给予蒸馏水灌胃、奥沙拉嗪灌胃、溃结饮灌胃和溃结饮灌肠。给药后7、14、28d取血,采用酶联免疫吸附法(ELISA)测定各组大鼠血清IL-4和IFN-γ的水平。结果溃结饮在灌胃与灌肠2种给药途径下均可显著降低血清IFN-γ、增加IL-4,从而调节IFN-γ/IL-4比值,抑制溃疡性结肠炎向Th1型细胞介导的炎症反应倾斜。急性期溃结饮灌胃作用好于灌肠(P<0.05),中、后期溃结饮灌肠作用好于灌胃,两者比较均具有统计学意义(P<0.05)。结论溃结饮2种给药途径对溃疡性结肠炎免疫机制调节的机制相同,急性期灌胃效果好于灌肠,中、后期灌肠效果好于灌胃,临床针对病程的不同阶段调整给药途径将会取得更好的疗效。
Objective To observe the interventional effects of two administration ways of Kuijie Decoction,gavage and enteroclysis,on the levels of serum interferon-γ（IFN-γ） and interleukin-4（IL-4） and ratio of IFN-γto IL-4.Methods All rats were randomly divided into the normal group（given distilled water） ,model group（given distilled water） ,olsalazine sodium control group（given olsalazine sodium） ,gavage group（intragastrical administration of Kuijie Decoction） and enteroclysis group（colonic administration of Kuijie Decoction） .The model was established by TNBS/ethanol method in all groups except the normal group（normal feeding） .After 7,14 and 28 days,the blood samples were collected respectively and the levels of serum IL-4 and IFN-γwere detected in rats of all groups by using ELISA.Results Kuijie Decoction administrated by two ways decreased significantly the level of serum IL-4 and increased the level of IFN-γ,regulated the ratio of IFN-γto IL-4,and inhibited ulcerative colitis（UC） tending to the inflammation mediated by Th1 cells.The effect of gavage was better than that of enteroclysis in acute stage of UC（P0.05） and vice versa in middle and late stage. The comparison showed a statistical significance（P0.05） .Conclusion Kuijie Decoction administrated by two ways has the same mechanism in the immunoregulation on UC.The effect of gavage was better than that of enteroclysis in acute stage and vice versa in middle and late stage.It will get better curativeeffect to change the administration way according to the stages of the disease.
出处
《北京中医药大学学报》
CAS
CSCD
北大核心
2010年第7期468-471,共4页
Journal of Beijing University of Traditional Chinese Medicine
基金
国家自然科学基金资助项目(No.30572390)
国家中医药管理局中医药科学技术研究基金资助项目(No.国中医药科02-03GP03)
