摘要
目的了解德国小蠊乙酰胆碱酯酶(AChE)活性的体躯分布和亚细胞分布。方法利用不同体躯和不同亚细胞的AChE活性进行测定。结果德国小蠊雄成虫的AChE在头部、胸部及腹部的活性分别占总活性的56.9%、19.5%和23.6%。德国小蠊的AChE在头、胸、腹间的活性分布差异有统计学意义(F=127.96,P<0.05),头部AChE的活性显著高于胸部和腹部(P<0.001),德国小蠊亚细胞AChE的总活性和比活力按大小排列依次为:线粒体>微粒体>胞质溶液>细胞核及细胞碎片。结论德国小蠊的AChE在头部、胸部、腹部均有分布,但头部活性最高,占整体活性的50%以上。亚细胞分布显示德国小蠊AChE在线粒体里分布最多,约占总活性的50%。
Objective To identify the body and subcellular distribution of acetylcholinesterase（AChE） activities in the Blattella germanica.Methods The AChE activities in different body parts and subcells were determined.Results The AChE activities in the head,chest and abdomin of male adults were 56.9%,19.5% and 23.6% of the total activity,respectively.The difference in activity between the three body parts was statistically significant（F=127.96,P0.05）,where the AChE activity in the head was significantly higher than those in the chest and abdomen（P0.001）.The subcellular organelles in descending order of the total and specific AChE activity were：mitochondriamicrosomescytoplasmic solutionnuclei and cell debris.Conclusion AChE activities were distributed in the head,chest and abdomen of B.germanica,with the highest in the head accounting for more than 50% of the overall activity.Subcellular distribution showed the highest AChE activity distribution in the mitochondria,accounting for almost 50% of the total activity.
出处
《中国媒介生物学及控制杂志》
CAS
CSCD
2010年第4期312-314,共3页
Chinese Journal of Vector Biology and Control
关键词
德国小蠊
乙酰胆碱酯酶
体躯分布
亚细胞分布
Blattella germanica
Acetylcholinesterase
Body distribution
Subcellular distribution
