摘要
目的 :探讨细胞凋亡与小肠粘膜萎缩发生之间的关系。材料和方法 :采用原位末端标记、免疫组化和 RT-PCR的方法 ,对正常小肠粘膜上皮和萎缩小肠粘膜上皮细胞凋亡的发生及其相关基因的表达进行对比研究。结果 :萎缩小肠粘膜上皮细胞凋亡的发生率明显高于正常小肠 ( P<0 .0 5) ;C- MYC在萎缩小肠绒毛顶部异常高表达 ;BAX和 BCL- 2呈弥散分布 ,BCL- 2在萎缩小肠粘膜上皮的表达显著低于正常小肠 ( P<0 .0 5) ,BAX的表达虽无显著性差异 ,但 BCL- 2与 BAX的比值明显降低。萎缩小肠粘膜上皮 bcl- 2的 m RNA表达较正常小肠明显降低 ,而 c-myc的 m RNA表达明显增高。结论 :小肠粘膜萎缩的发生与其细胞凋亡发生率增高有关 ,而细胞凋亡的增多与 bcl-2和 c-
Objective:To explore the relationship between apoptosis and intestinal mucosal atrophy. Method: A contrasting study on the occurrence and the distribution of apoptosis and the expression of apoptotic related genes between the normal and the atrophic intestinal mucosal epithelia was made by using the TUNEL(terminal deoxynudeotidy L transfere mediated dUTP biotin nick end ladeling) method, the immunohistochemical staining and reverse transcription PCR(RT PCR) method. Results: The rate of the apoptosis in the atrophic intestinal mucosal epithelia was significantly greater(P<0.05) than that in the normal intestine; the expression of C MYC was unusually increased in the atrophic intestinal villous tops; the distribution of BAX and BCL 2 was spread all over the epithelia and the expression of BCL 2 was significantly reduced (P<0.05) in atrophic intestine as compared with that in the normal intestine; there was no significant difference between the atrophic and the normal intestine in BAX expression, but the ratio of BCL 2 to BAX in the atrophic intestine was sinificantly reduced(P<0.05). Bcl 2 mRNA expression was more significantly reduced in the atrophic intestinal mucosa than that in the normal intestine, but c myc mRNA expression was significantly increased. Conclusion: It demonstrated that the occuring of the intestinal mucosal atrophy was closely related with the increasing of the apoptosis, and the increasing of the apoptosis was closely related with the abnormal expression of apoptosis related genes.
出处
《解剖学杂志》
CAS
CSCD
北大核心
1999年第2期118-123,共6页
Chinese Journal of Anatomy
基金
国家教委博士点基金!(No.9736)
上海市卫生局科技发展基金! (No.97429)资助
