摘要
将水稻白叶枯病原菌Xanthomonas oryzae pv.oryzae编码harpinXoo蛋白的hpa1Xoo基因构建植物重组表达质粒pCAMBIA3300-hpa1Xoo-bar,由根癌农杆菌Agrobacterium tumefa-ciensLBA4404介导,叶盘法转化寒菊QX-077,获得了草丁膦转基因寒菊株系。经PCR、Southernblot和RT-PCR检测证明hpa1Xoo已整合到寒菊基因组中。在转基因株系的叶片和植株上抗虫性鉴定表明,转基因寒菊显著抑制桃蚜Myzus persicae的繁殖(t<0.01)。RT-PCR表明转基因植株中hpa1Xoo能够在转录水平正常表达。hpa1Xoo转化寒菊可以正常表达并提高寒菊对蚜虫的抗性。
The gene hpa1Xoo encoding harpinXoo protein derived from Xathomonas oryzae pv.oryzae was inserted into transgenic vector pCAMBIA3300-bar.The leaf discs from chrysanthemum variety ＇Han jv＇ QX-077 were infected by Agrobacterium tumefaciens strain LBA4404/pCAMBIA3300-hpa1Xoobar.Basta-resistant green shoots were obtained.PCR,Southern blot and RT-PCR analyses confirmed successful integration of the foreign gene into the genome of the ＇Han jv＇ QX-077.Resistance bioassays of the leaves and plants of transgenic lines revealed that the transgenic plants could significantly inhibit the proliferation of Myzus persicae（t0.01）.RT-PCR analysis confirmed that hpa1Xoo had expressed in transgenic ＇han jv＇ plants at transcription level.Transformed chrysanthemum with hpa1Xoo gene could enhance the capability of aphid resistance.
出处
《中国生物防治》
CSCD
北大核心
2010年第3期280-286,共7页
Chinese Journal of Biological Control
基金
国家自然科学基金(30230240)
国家重点基础研究发展规划资助项目(G2006CB101902)
