摘要
用流式细胞仪、光镜、扫描电镜和透射电镜观察单纯放射损伤（单放组）、单纯烧伤（单烧组）和放射复合伤（复合伤组）小鼠骨髓基质细胞的细胞周期、ＤＮＡ含量和形态变化。结果：①单烧组骨髓基质细胞的Ｇ２＋Ｍ期比例、ＤＮＡ含量由高到低；单放组和复合伤组Ｇ２＋Ｍ期比例、ＤＮＡ含量则由低到高；②与正常组骨髓基质细胞相比，各损伤组均出现不同特征的形态学变化；③单放组的内质网扩张，线粒体基质变淡，嵴消失；单烧组的线粒体增多、肿胀；放射复合伤的线粒体重度退行性变，结构紊乱。提示：放射复合伤骨髓基质细胞的细胞周期比例、ＤＮＡ含量和形态的改变可能是导致放射复合伤造血微环境损伤的重要原因之一。
The dynamic changes of cell cycle and DNA content and morphology for the bone marrow stromal cells were assayed by flow cytometry, light microscope and electron microscope separately. The results showed: ①The bone marrow stromal cells occurred different types of morphology from mice treated with 5.0Gy γ ray radiation, 15% of total body surface area(TBSA) with Ⅲ degree burn injury, and combined irradiation burn injury respectively compared with that of controls. ②The transmission electron microscope revealed endoplasmic reticula were dilated, the matrix of mitochondria became pale and the cristae of mitochondria shorter in stromal cells from irradiation mice compared with those of controls. However, the mitochondria could be seen in stromal cells of burn injured mice. The structure of stromal cells showed vacuolation change, the mitochondria dilated and the structure of mitochondria from combined radiation burn injured mice appeared severe degeneration and disorder. ③The ratio of G 2+M phase and DNA content from stromal cells in irradiation mice and in combined radiation burn injured mice showed decreased. Otherwise, the same parameters from burn injured mice revealed contrary tendency compared with those of irradiation mice and combined radiation burn injured mice. The change in cell cycle, DNA content and morphology for bone marrow stromal cells was one of impairment injury mechanisms of bone marrow hematopoietic inductive microenvironment from mice post combined radiation burn injury.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
1999年第1期10-14,共5页
Medical Journal of Chinese People's Liberation Army
基金
军队医药卫生"九.五"指令性攻关课题
关键词
辐射损伤
烧伤
骨髓细胞
细胞周期
DNA
radiation injuries
experimental
burns
bone marrow cells
cell cycle
DNA
microscopy
electron
scanning
