摘要
目的观察凋亡抑制蛋白survivin在乳腺癌组织中的表达,及其与c-erbB-2、Ki-67增殖指数的相关性及临床病理意义。方法应用免疫组织化学SP法检测survivin在正常乳腺组织(20例)和乳腺癌组织(96例)中的表达以及Ki-67、c-erbB-2在乳腺癌组织中的表达。结果 survivin阳性表达乳腺癌的Ki-67增殖指数(35.32±21.28)%明显高于survivin阴性者(20.42±11.34)%,survivin表达与肿瘤细胞增殖呈正相关(P<0.01);survivin在乳腺癌组织中的阳性表达率为70.83%(68/96),正常乳腺组织未见survivin表达;survivin蛋白的表达与c-erbB-2的表达呈正相关(P<0.01);sur-vivin蛋白的表达与临床分期、淋巴结转移和5年生存率有关(P<0.05),与年龄、是否绝经、肿瘤大小和组织学分级均无关。结论 survivin不仅参与凋亡的调控,还促进了细胞增殖,在乳腺癌发生、发展中起重要作用,过度表达提示预后不良。
Objective To study the expression of inhibitor apoptosis protein survivin in breast carcimoma and its correlation with,c-erbB-2 expression and cell proliferation index and its clinicopathological significance.[WTHZ]Methods Immunohistochemistry（SP method） was adopted to detect survivin protein in the tissues of normal mammary （20 cases）,breast carcinoma （96 cases）and Ki-67,c-erbB-2 expressions in the breast carcinoma.Results The proliferation index in survivin-positive breast carcinoma （35.32±21.28）% was significantly higher than in survivin-negative breast carcinoma （20.42± 11.34 ）% （P〈0.05）,positive correlation between survivin expression and proliferative activity of tumor cell measured by Ki-67 index was found.The positive rate of survivin in breast carcinoma was 70.83%（61/96）,but no expression of survivin was detected in normal mammary tissues.surivivin expression was positively correlated with c-erbB-2（P〈0.01）.surivivin expression was related to clinic stage and lymph node metastasis and to 5 year survival rate.（P〈0.05）,but not to age,menopause,tumor size and histological grade.Conclusion survivin is not only involved in control of apoptosis,but also accelerates proliferation.survivin gene may play an important role in tumorigenesis and progression of breast carcinoma.survivin over expression indicates worse prognosis and can be used to evaluate the prognosis as a potential index.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
2010年第7期773-776,共4页
Cancer Research on Prevention and Treatment
