摘要
目的:观察乳腺癌新辅助化疗后不同时间肿瘤细胞Ki-67表达水平的变化情况,研究乳腺癌新辅助化疗后肿瘤细胞增殖变化的规律,探讨依此指导选择化疗最佳间期的可行性。方法:将乳腺癌组织接种于裸鼠前腿根部皮下,待其成活并生长到0.3～0.4 cm时,随机分为8组,取其中1组肿瘤组织应用免疫组化法测定肿瘤细胞Ki-67表达水平,其余7组行CAF(环磷腺胺、多柔比星和5-氟尿嘧啶)方案化疗1次,分别在化疗后1、3、5、7、14、21和28 d取肿瘤组织,应用免疫组化法测定肿瘤细胞Ki-67表达水平。结果:乳腺肿瘤细胞Ki-67表达水平从化疗后第3天开始逐渐降低,至化疗后14 d时降到最低点,然后又逐渐升高,至化疗后28 d时已接近化疗后第5天水平。结论:乳腺癌新辅助化疗后肿瘤细胞的增殖有其特定的变化规律,应用免疫组化法测定化疗后肿瘤细胞Ki-67表达水平,有可能指导临床选择最佳化疗间期,从而提高乳腺癌新辅助化疗效果。
OBJECTIVE： To observe the change of the level of the Ki-67 gene in different time of breast carcinomas after preoperative chemotherapy,investigate the disciplinarian of proliferation and analyze the feasibility of chemotherapy time.METHODS： The breast fat pads of mude mice were inoculated with the survival breast tissue.When the pads grew to 0.3-0.4 cm,they were randomly divided into eight groups.Seven groups of that had chemotherapy of CAF scheme（cyclophosphamide,arubicin,and 5-fluorouracil）.The expression of Ki-67 of tumor cells was detected by immunohistochemistry in the rest of one group,and in the chemotherapy-groups 1,3,5,7,14,21 and 28 days after the chemotherapy.RESULTS： The Ki-67 expression levels of breast cancer cells gradually lowered from the first three days after the chemotherapy,to bottom after 14 days,then gradually increased after 28 days,reaching nearly the level of 5 days after the chemotherapy.CONCLUSIONS： Breast cancer cell proliferation after neoadjuvant chemotherapy has its particular variation.Application of the immunohistochemical determination method for detecting Ki-67 expression levels of tumor cells after chemotherapy,may guide the best chemotherapy,thereby improving the interphase effect of breast cancer neoadjuvant chemotherapy.
出处
《中华肿瘤防治杂志》
CAS
2010年第13期976-978,共3页
Chinese Journal of Cancer Prevention and Treatment
基金
首都医学发展科研基金资助项目(2002-3095)
