摘要
采用荧光光谱分析法,研究了饮用水消毒副产物三氯甲烷、三溴甲烷与人血丙种球蛋白之间的相互作用.结果表明,三氯甲烷对人血球蛋白产生动态荧光猝灭,而三溴甲烷对人血球蛋白的内源荧光有静态猝灭作用.Fe3+、Al3+和聚丙烯酰胺的加入也使人血丙种球蛋白的荧光强度降低,结合的强弱依次为:聚丙烯酰胺>Fe3+>Al3+.在二元体系的基础上,进一步研究了Fe3+、Al3+和聚丙烯酰胺对CHBr3-人血丙种球蛋白结合作用的影响.结果表明,Fe3+、Al3+的存在使CHBr3-人血丙种球蛋白的结合常数呈现降低的趋势,聚丙烯酰胺对其基本无影响.
The interaction between chloroform,tribromethane and gamma seroglobulinum humanum（GSH）was investigated by using fluorescence spectroscopy method. The fluorescence intensity of GSH was quenched when chloroform and tribromethane were added. The quenching mechanism of GSH by chloroform was a dynamic quenching procedure,while tribromethane was a static quenching procedure. Fe^3＋,Al^3＋and polypropylene also separately quenched fluorescence intensity of GSH,and the binding intensity of them with GSH is polypropyleneFe^3＋Al^3＋. The presence of Fe3＋ and Al^3＋ in the binary system of tribromethane-gamma seroglobulinum humanum reduced the binding constant of CHBr3-GSH,but polypropylene almost has no influence on it.
出处
《环境化学》
CAS
CSCD
北大核心
2010年第4期665-668,共4页
Environmental Chemistry
