摘要
为了解巴布亚新几内亚地方鸡群体的遗传多样性和亲缘关系,本研究利用20个微卫星DNA遗传标记,对3个群体、96个个体的基因型进行了测定,估算了群体内的杂合度和近交系数(FIS)以及群体间的遗传分化系数(FST)等多样性参数;根据Nei氏标准遗传距离,构建群体水平的NJ树和个体水平上的无根UPGMA树。结果表明,3个群体内的平均期望(基因)杂合度介于0.665 9至0.680 6,平均FIS介于0.020至0.286,总群体水平上的遗传变异仅占5.5%;3个群体相互之间的遗传分化均达到显著水平,在个体水平的无根UPGMA树上,来源同一群体的个体会优先聚为一类。这说明巴布亚新几内亚的3个地方鸡群体的遗传多样性比较丰富,群体间的遗传分化显著,应该分别加以保护和利用。
To assess the genetic diversity within indigenous chicken populations from Papua New Guinea（PNG） and the relationship among them,20 microsatellite DNA markers were used to genotype 96 samples of three PNG chicken populations.The heterozygosity and inbreeding coefficient（FIS） within the populations and genetic differentiation coefficient（FST） among the populations were estimated among other diversity parameters.NJ tree at population level and un-rooted UPGMA trees at individual level using Nei′s standard genetic distances were reconstructed to examine the genetic relationships among the populations.The estimates of average expected（gene） heterozygosity and FIS within the three populations ranged between 0.665 9 and 0.680 6,and between 0.020 and 0.286,respectively.The genetic differentiation among populations was 5.5% of total genetic variability.Majority of the individuals clustered into different groups in the un-rooted UPGMA tree following their origins of sampling.The three Papua New Guinean indigenous chicken populations had a high level of genetic diversity within the populations and significant genetic differentiations among the populations,which called for specific attention on these unique genetic resources in the conservation and utilization programs.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2010年第7期797-803,共7页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
"十一五"国家科技支撑计划项目(2008BADB2B01)
CAAS-ILRI联合研究项目
中国农业科学院一级岗位杰出人才基金资助
关键词
巴布亚新几内亚
地方鸡
微卫星
遗传多样性
Papua New Guinea
indigenous chicken
microsatellite
genetic diversity
