摘要
目的：探讨ＮＫ细胞杀伤与靶细胞表面ＭＨＣＩ类分子的关系。方法：用ＨＬＡＡＢＣ单抗培养上清或腹水封闭靶细胞Ｋ５６２表面的ＨＬＡＡＢＣ分子后，分别观察了外周血新鲜ＮＫ细胞、纯化ＮＫ细胞、ＬＡＫ细胞和ＣＤ３ＡＫ细胞对Ｋ５６２细胞杀伤能力的变化。结果：外周血新鲜ＮＫ细胞和纯化ＮＫ细胞对Ｋ５６２的杀伤率明显提高，ＣＤ３ＡＫ细胞的杀伤力明显降低，ＬＡＫ细胞的杀伤无明显变化。结论：ＮＫ细胞通过其表面受体识别靶细胞上的ＭＨＣＩ类分子而传递杀伤抑制性信号，抗ＨＬＡＡＢＣ单抗封闭了靶细胞上的ＨＬＡＡＢＣ等位基因而阻止了阴性信号的传导。
Objective:To study the relation of cytolytic activity of NK cells and MHC class I molecules on target cells.Metheds:The changes of cytolytic activity of fresh NK cells seperated from peripheral blood,purified NK cells,LAK cells and CD3AK cells were observed when target cells were cultured with anti HLA ABC monoclonal antibody for 30 minutes.Results:The cytolytic activity of fresh NK cells and purified NK cells from LAK cells was highly increased,but that of CD3AK cells was lowered.The cytotxicity of LAK cells was not affected.Conclution:NK cell can recognize the MHC class I molecules on target cells and then inhibit its cytolytic activity by transmitting kill inhibitory signals.The anti HLA ABC monoclonal antibody had prevented the delivering of negative signals by blocking the MHC class I molecules on target cells.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
1999年第3期112-113,116,共3页
Chinese Journal of Immunology
