摘要
目的探讨慢性乙型肝炎外周血自然杀伤细胞(NK细胞)和活化NK细胞数量的变化及其与HLA-Cw基因型的关系。方法采用流式细胞术检测30例急性乙型肝炎发病期患者、51例慢性乙型肝炎患者及41例正常对照外周血NK细胞和活化NK细胞的数量,并通过序列特异性寡核苷酸分型(PCR-SSO)技术进行HLA-Cw的基因分型。结果急性肝炎组的NK细胞和活化NK细胞的数量均较正常对照组增高[(22.62±3.70)%vs(19.03±4.91)%,(65.28±14.45)%vs(53.88±14.01)%,P<0.05],慢性乙型肝炎组的NK细胞和活化NK细胞的数量均较正常对照组降低[(14.36±1.07)%vs(19.03±4.91)%,(47.14±5.67)%vs(53.88±14.01)%,P<0.05],且急性肝炎组与慢性乙型肝炎组间差异有统计学意义(P<0.01);急性肝炎组与正常对照组间HLA-Cw位点各等位基因的基因型无统计学差异(P>0.05),HLA-Cw*03在慢性乙型肝炎组中基因频率明显高于正常对照组(P<0.05),且与活化NK细胞数量呈显著负相关(γ=-0.394,P<0.05)。结论慢性乙型肝炎患者NK细胞功能低下,HLA-Cw*03基因型可能是通过影响NK细胞而导致HBV感染持续存在的原因之一。
Objective To investigate the correlation between the frequency of circulating natural killer cells （NK cells）and HLA- Cw alleles. Methods 30 patients with acute hepatitis B in nonage and 51 patients with chronic hepatitis B were included in the experimental groups, and 41 healthy individuals were enrolled as controls. Numbers of circulating NK cells and activated NK cell were analyzed by flow cytometry. HLA-Cw genotyping was conducted by the polymerase chain reaction sequence specific ohgonucleotide （PCR-SSO） method. Results Numbers of circulating NK cells and activated NK cell in acute hepatitis B patients were higher than in healthy subjects[ （22.62 ± 3.70） % vs （ 19.03 ± 4.91 ） %, and （65.28 ± 14.45） % vs （53.88 ± 14.01 ） %, both P〈0.05], however, those in patients with chronic hepatitis B were lower than in healthy subjects[ （14.36 ± 1.07）% vs （19.03±4.91）%, and （47.14±5.67）% vs （53.88± 14.01）%, both P〈0.05], there were statistically significant differences between the groups of chronic hepatitis B and acute hepatitis B（ P 〈 0.01 ）. There was no statistical significance between the groups of acute hepatitis B and healthy subjects about HLA-Cw（ P 〉 0.05. HLA-Cw^＊ 3 was more frequent in patients with chronic hepatitis B than in controls （ P 〈 0.05 ）, and there was a significantly negative correlation between the frequency of HLA-Cw^＊ 03 and the numbers of activated NK cell in the group of chronic hepatitis B（ γ = - 0. 394, P 〈 0.05）. Conclusions function of NK cells in chronic hepatitis B is weak. The HLA-Cw^＊ 03 gene may affect the antiviral function of NK cells to induce the persistence of HBV infection.
出处
《山东大学学报（医学版）》
CAS
北大核心
2008年第7期697-700,共4页
Journal of Shandong University:Health Sciences
基金
山东省卫生厅科研基金资助课题(2005HZ007)
