摘要
为了解慢性ＨＢＶ携带者体内ＨＢＶ病毒的状况，以选择ＨＢＶ前Ｓ１／Ｓ２为研究区段，应用半巢式ＰＣＲ法从３例慢性ＨＢＶ携带者血清中扩增出ＨＢＶ前Ｓ１／Ｓ２基因，分别将其克隆于噬菌体Ｍ１３ｍｐ１８、Ｍ１３ｍｐ１９，每例随机筛选１０个阳性克隆，进行序列分析及遗传距离分析，结果发现：３例病人各克隆分别与同源性最好的ＨＢＶ野生株相比，存在着明显的碱基替代、缺失和插入。此类病人体内的ＨＢＶ株同源性差，克隆间最大的遗传距离为０．１２５。慢性ＨＢＶ携带者体内的ＨＢＶ株呈“相似株”分布。
To investgate the HBV in chronic HBsAg carriers,the preS1/S2 were selected as studied regions Half nested polymerase chain reaction (PCR)was used to amplify HBV preS1/S2 gene from sera of 3 chronic HBsAg carriers.PCR products were cloned into M13mp18、M13mp19 vector for DNA sequencing.Results showed that compared with the wild type HBV adr,all clones exhibited base insertion and substitution.The biggest genetic distance among those clones was 0.125.These data confirmed that quasispecies were the major characteristics in these chronic HBsAg carries.
出处
《免疫学杂志》
CAS
CSCD
北大核心
1999年第2期113-116,共4页
Immunological Journal
基金
国家自然科学基金
