摘要
目的应用微导管介入技术建立由冠状动脉微栓塞所致的微血管功能障碍的动物模型。方法10只巴马系小型猪经导管选择性地于左前降支动脉（left anterior descending artery,LAD）内注入微栓塞球（Φ45μm）,致使冠状动脉微血管栓塞。分别测量不同微球剂量（5×、10×、12×、15×、18×104）下的冠脉血流储备分数（coronary flow reserve,CFR）,冠脉血流量（coronary blow flow,CBF）,左室射血分数（ejection fraction,EF）及冠状窦内内皮素（endothelin-1,ET-1）的浓度。动物处死后心肌组织切片行HE染色。结果冠脉内（10~15）×104的微球量可导致CBF及血流储备的降低（CFR〈2.0）以及左室功能障碍的发生（EF〈50%）。冠状窦内ET-1的异常变化同组织切片HE染色均证实微血管完整性的破坏。结论应用微导管介入技术可建立创伤小、死亡率低的冠状动脉微血管功能障碍的动物模型。是临床研究冠脉微血管栓塞致微血管功能障碍发病机制的理想模型。
Objective To establish a coronary microvascular dysfunction（CMD） animal model induced by coronary microembolization（CME） using percutaneous endovascular technique in miniswine. Methods CMD in miniswines induced by selective infusion microspheres（45 μm） into the left anterior descending artery（n=10）.At different doses of microspheres（5×,10×,12×,15×,18×104）,coronary average flow velocity and lumen area were measured using Doppler flow wire and intracoronary ultrasound imaging catheter in the middle segment of left anterior descending artery（LAD）.Coronary blood flow（CBF） was calculated from the equation of flow velocity times lumen area.Intracoronary bolus injection of adenosine was used to induce maximal coronary hyperemic reaction.Coronary flow reserve（CFR） was defined as the ratio of hyperemic CBF to resting CBF.Left ventricular ejecting fraction（EF） was assessed by echocardiography,and endothelin-1（ET-1） level in coronary sinus was measured by radioimmunioassay.HE staining was used to demonstrate the presence of microembolization after experimental protocol. Results At the doses of（10-15）×104 microspheres,coronary microvascular integrity（CFR2.0）,left ventricular function（EF50%） was damaged,and ET-1 level was increased significantly by CME.HE staining demonstrated the presence of microembolization after CME. Conclusions The CMD miniswine model is characterized by minimally-invasive,technically-simple with low mortality.Therefore,it is an ideal animal model for investigating mechanism of human CMD.
出处
《复旦学报（医学版）》
CAS
CSCD
北大核心
2010年第4期450-454,共5页
Fudan University Journal of Medical Sciences
基金
上海市浦江人才计划(2008D类)项目
关键词
微血管功能
微栓塞
血管内介入
动物模型
小型猪
microvascular function
microembolization
endovascular technique
animal model
miniswine
