摘要
目的:探讨中药干预糖尿病胆石形成的作用机制。方法:60只实验兔随机分为正常组(A组n=10),糖尿病组(B组n=12),糖尿病+中药1号组(C组n=14),糖尿病+中药2号组(D组n=12),糖尿病+中药3号组(E组n=12)。B、C、D、E组首先制备糖尿病模型,维持非禁食状态血糖在20mmol/L左右。A、B组每日1次给予0.9%生理盐水4.2ml/kg胃管灌注;C、D、E组每日1次分别给予中药1号(大黄、郁金、茵陈),中药2号(大黄、柴胡、茵陈),中药3号(大黄、郁金、柴胡、茵陈)4.2ml/胃管灌注。6周后取血测血脂;断头处死,测胆汁中胆固醇/磷脂比值及胆汁酸成分;观察胆囊成石情况及电镜下超微结构的变化。结果:3个中药组显著降低糖尿病兔的血胆固醇(t1=5.13,t2=5.232,t3=5.14;P<0.01)、血甘油三酯(t1=3.45,t2=3.24,t3=3.02;P<0.01);降低胆汁中胆固醇/磷脂比值(t1=5.29,t2=5.18,t3=6.36;P<0.01);降低胆汁中胆酸/鹅去氧胆酸比值(t1=9.047,t2=8.627,t3=9.339;P<0.01);减少去氧胆酸生成(t1=6.839,t2=7.492,t3=9.045;P<0.01);有效改善糖尿病兔胆囊超微结构;降低胆囊成石评分(t1=5.66,t2=7.74,t3=7.93;P<0.01);3个中药组中尤以中药3号对胆酸/鹅去氧胆酸比值、去氧胆酸含量改善最明显(f=3.699,P<0.05;f=7.83,P<0.01),对胆囊超微结构的改善效果也最为突出,胆囊成石评分低于其余两组,但无统计学差异(f=0.248,P>0.05;)。结论:中药可以有效干预糖尿病兔胆囊胆固醇结石的形成过程,大黄、柴胡、茵陈、郁金四药联合应用效果较佳。
Objective：To observe the effect of Traditional Chinese Herbal Medicine on the forming of gallstones induced by diabetic rabbits,illustrating the mechanism of it and conclude the best herb collocation.Methods：Sixty mature white rabbits（male and female are equal）were randomly divided into five groups：group A is normal group,group B is diabetes group,group C is diabetes with group 1 treated by chinese herbs,group D is diabetes with group 2 treated by chinese herbs,group E is diabetes with group 3 treated by chinese herbs.Blood sugar of group A is normal and the random blood sugar of the other four groups was controlled near 20 mmol/L.The five groups were all fed with common feedstuff.Group A and B were treated with NS（4.2 ml/kg）pouring into stomach one time a day,the other three groups were treated with herbs（4.2ml/kg）.After feeding 6 weeks,taking blood from auditive vein to measure the blood CHO、TG、HDL、LDL,then killing them by cutting the heads,peeling off gallbladder,drawing out the bile and observing the bilestones on the dialyzer.Measuring the cholesterol、phospholipase、bile acid and analyzing the elements of the bile acid.Fetching new cholecystic tissues and observe the changes of ultrastructure.Results：The three herb groups were compared with the diabetes group：the ratio of gallstones is lower（P〈0.01）;the blood CHO、TG、LDL descends obviously（P〈0.01）;the cholesterol and DCA in the bile are low while the phospholipase and CDCA are high（P〈0.01）;the damage on microvascular change and cholecystic epidemis wasn′t as serious as the DM group.Comparing between the three herb gropus：there is the most obvious improvement of the bile acid composition in group 3,and the change of ultrastructure in group 3 is the lightest and the most near normal.Conclusions：Chinese Medicine can effective intervention diabetic rabbit′s cholesterol gallstone formation process,especially the combination of the four kinds of herbs.
出处
《四川中医》
2010年第7期17-20,共4页
Journal of Sichuan of Traditional Chinese Medicine
基金
天津市卫生局资助课题(项目编号:027~02012)
关键词
中药
糖尿病动物
胆固醇结石
Traditional Chinese Herbal Medicine diabetes animals cholesterol gallstones
