摘要
以贝克桉(Eucalyptusbaker)无菌苗的半木质化带未萌发腋芽的茎段为试验材料,用不同消毒处理对贝克桉外植体进行灭菌,并进行愈伤组织及丛生芽产生的初步研究。结果表明:贝克桉茎段外植体洗刷干净后使用75%乙醇溶液灭菌30 s,无菌水冲洗3次,每次3 min;后再用0.1%HgCl2分二步消毒,每次处理2 min,每次消毒后用无菌水冲洗3次,每次3 min。这种处理方法污染率相对较低,褐变程度最小。在改良MS+6-BA 0.2 mg/L+IBA 0.2 mg/L+蔗糖30 g/L+卡拉胶6 g/L+AC 2 g/L的培养基上培养1个月后可诱导贝克桉茎段愈伤组织的生成。
Taking the non-vaccine＇s half lignification belt of not sprouted the axial bud and the stem section of Eucalyptus baker,the pile cutted down with the spiral spring and the no-sprouteing axial bud of the branch of Eucalyptus dunnii as the material,processed with the different disinfection to the explant of Eucalyptus baker to carry on antiseptically,and carried on the preliminary study of recovering the wound to organize and to grow thickly which the bud produces.The result indicated：after the explant of Eucalyptus baker scrubbing cleanly,used 75% ethyl alcohol solution antiseptic 30 s,the sterilized water flushed three times,each time 3 minutes;Latter used 0.1% HgCl2 to divide two steps again to disinfect,each time processed 2 minutes,after each time disinfecting,flushed with the sterilized water three times,each time 3 minutes.the pollution rate of this kind of processing method was relatively low,the browning degree was the lowest.Raised one month later,the culture medium of the improved MS＋6-BA 0.2 mg/L＋IBA 0.2 mg/L＋sucrose 30 g/L＋Kara rubber 6 g/L＋AC 2 g/L might induce the production of the injury-organization of the stem section from Eucalyptus baker.
出处
《北方园艺》
CAS
北大核心
2010年第13期151-153,共3页
Northern Horticulture
关键词
贝克桉
灭菌方法
褐变
Eucalyptus baker
sterilization methods
browning
