摘要
目的探讨丹酚酸B盐(SA-B)对腹主动脉结扎大鼠心肌神经内分泌因子及间质性胶原沉积的影响。方法腹主动脉不完全结扎法制备大鼠心肌肥厚模型。大鼠随机分为假手术组、模型组,丹酚酸B盐低、中、高剂量组和卡托普利组,各药物组均采用灌胃给药。测量大鼠收缩压(SBP)、舒张压(DBP),称重法测定心脏指数(HW/BW、LVW/BW);放免法测定心肌血管紧张素Ⅱ(AngⅡ)、血清醛固酮(ALD)含量,消化法测定心肌羟脯氨酸(Hyp)含量,免疫印迹法观察心肌Ⅰ、Ⅲ型胶原蛋白表达水平。结果模型组SBP、DBP、HW/BW、LVW/BW、AngⅡ、ALD、Hyp及Ⅰ、Ⅲ型胶原含量升高,心肌组织Ⅰ、Ⅲ型胶原蛋白表达明显增加。SA-B各用药组能改善心肌肥厚指数,降低AngⅡ及Hyp水平和Ⅰ、Ⅲ型胶原蛋白表达,以中、高剂量组作用更为显著。结论 SA-B能通过降低压力负荷性心肌肥厚大鼠肾素-血管紧张素-醛固酮系统活性而抑制心肌间质性纤维化的形成。
Objective To evaluate the influence of Salviandic acid B （SA-B） on some neurohormonal factors and interstitial collagen in ventricular remodeling induced by abdominal aortic banding （AAB） in rats. Method Ventricular remodeling model was induced by AAB in rats. Rats were randomly divided into 6 groups：normal, model, SA-B high, SA-B middle, SA-B low and Captopril control group. The drug groups used gavage method. The systolic blood pressure （SBP） and diastolic blood pressure （DBP） were measured, the ratios of LVW/BW （1eft ventricle weight/body weight）, HW/BW （heart weight/body weight） were calculated, the Angiotensin Ⅱ （Ang Ⅱ） in heart tissue and Aldosterone （ALD） concentration of plasma were checked by radioimmunoassay, hydroxy proline （Hyp） content in heart tissue was measured by hydrolysis method, the level of type Ⅰ and Ⅲ collagen in myocardium were determined by western blotting. Result SA-B could decrease the cardiac indexes of LVW/BW and HW/BW, significantly reduce the content of Ang Ⅱ activity, significantly down-regulate Hyp content and the level of type Ⅰ and Ⅲ collagen in myocardium. Conclusion SA-B can inhibit heart interstitial fibrosis in experimental ventricular remodeling by inhibiting Ang Ⅱ activity.
出处
《中国中医药信息杂志》
CAS
CSCD
2010年第7期46-48,共3页
Chinese Journal of Information on Traditional Chinese Medicine
基金
上海市教委资助项目(06CZ040)
上海市教委重点学科建设项目(Y0302-5)
